Abstract
Adult rat liver slices were maintained for 20 h in a novel organ culture system with minimal loss of viability and function. Potassium and adenosine triphosphate levels were maintained at in vivo levels, following an initial recovery period (2 to 4 h), for up to 20 h. Protein synthesis and secretion were linear for 20 and 16 h, respectively. In addition, the liver slices synthesized glycogen between 4 and 12 h in culture. Finally, the liver slices were hormonally responsive during the 20 h culture period as exemplified by glucagon-stimulated glucose production. This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function.
Original language | English (US) |
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Pages (from-to) | 706-712 |
Number of pages | 7 |
Journal | In Vitro Cellular & Developmental Biology |
Volume | 22 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1986 |
Keywords
- hormone stimulation
- liver slices
- organ culture
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology