Maintenance of adult rat liver slices in dynamic organ culture

P. F. Smith, G. Krack, R. L. McKee, D. G. Johnson, A. J. Gandolfi, V. J. Hruby, C. L. Krumdieck, K. Brendel

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Adult rat liver slices were maintained for 20 h in a novel organ culture system with minimal loss of viability and function. Potassium and adenosine triphosphate levels were maintained at in vivo levels, following an initial recovery period (2 to 4 h), for up to 20 h. Protein synthesis and secretion were linear for 20 and 16 h, respectively. In addition, the liver slices synthesized glycogen between 4 and 12 h in culture. Finally, the liver slices were hormonally responsive during the 20 h culture period as exemplified by glucagon-stimulated glucose production. This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function.

Original languageEnglish (US)
Pages (from-to)706-712
Number of pages7
JournalIn Vitro Cellular & Developmental Biology
Volume22
Issue number12
DOIs
StatePublished - Dec 1986

Keywords

  • hormone stimulation
  • liver slices
  • organ culture

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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