Lysis of human red blood cells 2: Effect of contact time on cosolvent induced hemolysis

Joseph F. Krzyzaniak; Dawn M. Raymond; Samuel H. Yalkowsky

doi:10.1016/S0378-5173(97)00082-3

Lysis of human red blood cells 2: Effect of contact time on cosolvent induced hemolysis

Joseph F. Krzyzaniak
, Dawn M. Raymond
, Samuel H. Yalkowsky

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.

Original language	English (US)
Pages (from-to)	193-200
Number of pages	8
Journal	International Journal of Pharmaceutics
Volume	152
Issue number	2
DOIs	https://doi.org/10.1016/S0378-5173(97)00082-3
State	Published - Jun 26 1997

Keywords

Cosolvent
Dynamic
Hemolysis
In vitro

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.1016/S0378-5173(97)00082-3

Cite this

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title = "Lysis of human red blood cells 2: Effect of contact time on cosolvent induced hemolysis",

abstract = "The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.",

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T1 - Lysis of human red blood cells 2

T2 - Effect of contact time on cosolvent induced hemolysis

AU - Krzyzaniak, Joseph F.

AU - Raymond, Dawn M.

AU - Yalkowsky, Samuel H.

PY - 1997/6/26

Y1 - 1997/6/26

N2 - The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.

AB - The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.

KW - Cosolvent

KW - Dynamic

KW - Hemolysis

KW - In vitro

UR - https://www.scopus.com/pages/publications/0030814161

UR - https://www.scopus.com/pages/publications/0030814161#tab=citedBy

U2 - 10.1016/S0378-5173(97)00082-3

DO - 10.1016/S0378-5173(97)00082-3

M3 - Article

AN - SCOPUS:0030814161

SN - 0378-5173

VL - 152

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JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 2

ER -

Lysis of human red blood cells 2: Effect of contact time on cosolvent induced hemolysis

Abstract

Keywords

ASJC Scopus subject areas

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