TY - JOUR
T1 - Lysis of human red blood cells 2
T2 - Effect of contact time on cosolvent induced hemolysis
AU - Krzyzaniak, Joseph F.
AU - Raymond, Dawn M.
AU - Yalkowsky, Samuel H.
PY - 1997/6/26
Y1 - 1997/6/26
N2 - The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.
AB - The degree of hemolysis induced by several cosolvent formulations is evaluated at various contact times using the dynamic in vitro method developed by Krzyzaniak (Krzyzanik, J.F., Raymond, D.M. and Yalkowsky, S.H., Lysis of Human Red Blood Cells 1: Effect of Contact Time on Water Induced Hemolysis, PDA J. Pharm. Sci. and Tech., 50 (1996) 223-226). Hemolysis is shown to increase with cosolvent concentration and to be sigmoidally related to the logarithm of the formulation:blood contact time. With this information, a physiologically realistic in vitro method with a formulation:blood ratio of 0.1 and a contact time of 1 s has been developed and used to estimate the amount of hemolysis occurring after an intravenous injection of some commonly used cosolvent formulations.
KW - Cosolvent
KW - Dynamic
KW - Hemolysis
KW - In vitro
UR - http://www.scopus.com/inward/record.url?scp=0030814161&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030814161&partnerID=8YFLogxK
U2 - 10.1016/S0378-5173(97)00082-3
DO - 10.1016/S0378-5173(97)00082-3
M3 - Article
AN - SCOPUS:0030814161
SN - 0378-5173
VL - 152
SP - 193
EP - 200
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 2
ER -