Lymphatic endothelial cell line (CH3) from a recurrent retroperitoneal lymphangioma.

D. Way, M. Hendrix, M. Witte, C. Witte, R. Nagle, J. Davis

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


An endothelial cell line derived from a massive recurrent chyle-containing retroperitoneal lymphangioma was isolated in monolayer culture. Scanning and transmission electron microscopy and immunohistochemistry confirmed a close resemblance to blood vascular endothelium with typical cobblestone morphology, positive immunofluorescence staining for endothelial marker Factor VIII-associated antigen and fibronectin, and prominent Weibel-Palade bodies. The endothelial cells also exhibited other ultrastructural features characteristic of lymphatic endothelium, including sparse microvillous surface projections, overlapping intercellular junctions, and abundant intermediate filaments. This endothelial cell line represents a new source of proliferating lymphatic endothelium for future study, including structural and functional comparison to blood vascular endothelium.

Original languageEnglish (US)
Pages (from-to)647-652
Number of pages6
JournalIn vitro cellular & developmental biology : journal of the Tissue Culture Association
Issue number9
StatePublished - 1987

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology


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