Ly-6I, a new member of the murine Ly-6 superfamily with a distinct pattern of expression

David L. Pflugh, Stephen E. Maher, Alfred L.M. Bothwell

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A new member of the mouse Ly-6SF, designated Ly-6I, has been isolated as a gene homologous to a segment of the Ly-6C gene. A single allelic difference in the mature protein sequence was identified, which is similar to other Ly- 6SF members. Ly-6I mRNA has been detected in a wide range of tissues and cell lines, and a rabbit polyclonal Ab has been used to determine that Ly-6I protein is present at a low constitutive level on cell lines from several different lineages. In contrast to Ly-6C and Ly-6A/E, the Ly-6I gene is only weakly responsive to IFNs. Expression in vivo is most abundant on bone marrow populations and is coexpressed with Ly-6C on granulocytes and macrophages. However, Ly-6I is also expressed on immature B cell populations that do not express Ly-6C. Expression on mature B cells in spleen is uniformly low. Similarly, Ly-6I is expressed on TCR(low/int), but not TCR(high), thymocytes. Ly-6I is re-expressed on Ly-6C(high) T cells in the periphery. Thus, Ly-6I may be a useful marker to define maturation stages of both T and B lymphocytes as well as subsets of monocytes and granulocytes.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalJournal of Immunology
Issue number1
StatePublished - Jul 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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