Lower resting and exercise-induced circulating angiogenic progenitors and angiogenic T cells in older men

Mark D. Ross, Eva M. Malone, Richard Simpson, Islay Cranston, Lesley Ingram, Graham P. Wright, George Chambers, Geraint Florida-James

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Aging is associated with a dysfunctional endothelial phenotype as well as reduced angiogenic capabilities. Exercise exerts beneficial effects on the cardiovascular system, possibly by increasing/maintaining the number and/or function of circulating angiogenic cells (CACs), which are known to decline with age. However, the relationship between cardiorespiratory fitness (CRF) and age-related changes in the frequency of CACs, as well as the exercise-induced responsiveness of CACs in older individuals, has not yet been determined. One-hundred seven healthy male volunteers, aged 18–75 yr, participated in study 1. CRF was estimated using a submaximal cycling ergometer test. Circulating endothelial progenitor cells (EPCs), angiogenic T cells (TANG), and their chemokine (C-X-C motif) receptor 4 (CXCR4) cell surface receptor expression were enumerated by flow cytometry using peripheral blood samples obtained under resting conditions before the exercise test. In study 2, 17 healthy men (8 young men, 18–25 yr; 9 older men, 60–75 yr) were recruited, and these participants undertook a 30-min cycling exercise bout at 70% maximal O2 consumption, with CACs enumerated before and immediately after exercise. Age was inversely associated with both CD34+ progenitor cells (r2=0.140, P = 0.000) and TANG (r2=0.176, P = 0.000) cells as well as CXCR4- expressing CACs (CD34+: r2=0.167, P = 0.000; EPCs: r2=0.098, P = 0.001; TANG: r2=0.053, P = 0.015). However, after correcting for age, CRF had no relationship with either CAC subset. In addition, older individuals displayed attenuated exerciseinduced increases in CD34+ progenitor cells, TANG, CD4+, TANG, and CD8+CXCR4+ TANG cells. Older men display lower CAC levels, which may contribute to increased risk of cardiovascular disease, and older adults display an impaired exercise-induced responsiveness of these cells. NEW & NOTEWORTHY Older adults display lower circulating progenitor cell and angiogenic T cell counts compared with younger individuals independently of cardiometabolic risk factors and cardiorespiratory fitness. Older adults also display impaired exercise-induced mobilization of these vasculogenic cells.

Original languageEnglish (US)
Pages (from-to)H392-H402
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume314
Issue number3
DOIs
StatePublished - Mar 2018

Keywords

  • Age
  • Angiogenesis
  • Exercise
  • Fitness
  • Progenitor cells
  • T cells

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Lower resting and exercise-induced circulating angiogenic progenitors and angiogenic T cells in older men'. Together they form a unique fingerprint.

Cite this