Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo

Nachiket Kulkarni; Andrew Masciola; Abhinav Nishant; Kyung Jo Kim; Heejoo Choi; Arthur Gmitro; Esther E. Freeman; Aggrey Semeere; Miriam Nakalembe; Dongkyun Kang

doi:10.1364/BOE.434892

Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyung Jo Kim, Heejoo Choi, Arthur Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, Dongkyun Kang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.

Original language	English (US)
Pages (from-to)	5629-5643
Number of pages	15
Journal	Biomedical Optics Express
Volume	12
Issue number	9
DOIs	https://doi.org/10.1364/BOE.434892
State	Published - Sep 1 2021

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.434892

Cite this

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title = "Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo",

abstract = "We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.",

author = "Nachiket Kulkarni and Andrew Masciola and Abhinav Nishant and Kim, {Kyung Jo} and Heejoo Choi and Arthur Gmitro and Freeman, {Esther E.} and Aggrey Semeere and Miriam Nakalembe and Dongkyun Kang",

note = "Funding Information: Acknowledgment. This research was supported by the National Institutes of Health (R21TW011222). Publisher Copyright: {\textcopyright} 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.",

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doi = "10.1364/BOE.434892",

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AU - Kulkarni, Nachiket

AU - Masciola, Andrew

AU - Nishant, Abhinav

AU - Kim, Kyung Jo

AU - Choi, Heejoo

AU - Gmitro, Arthur

AU - Freeman, Esther E.

AU - Semeere, Aggrey

AU - Nakalembe, Miriam

AU - Kang, Dongkyun

N1 - Funding Information: Acknowledgment. This research was supported by the National Institutes of Health (R21TW011222). Publisher Copyright: © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

PY - 2021/9/1

Y1 - 2021/9/1

N2 - We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.

AB - We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.

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DO - 10.1364/BOE.434892

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EP - 5643

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 9

ER -

Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo

Abstract

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