We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
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Kulkarni, N. (Creator), Masciola, A. (Creator), Nishant, A. (Creator), Kim, K. (Creator), Choi, H. (Creator), Gmitro, A. F. (Creator), Freeman, E. E. (Creator), Semeere, A. (Creator), Nakalembe, M. (Creator) & Kang, D. (Creator), The Optical Society, 2021