Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyung Jo Kim, Heejoo Choi, Arthur Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, Dongkyun Kang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 μm and 4 μm for lateral and axial directions, respectively. Effective field size was 468 μm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 μm.

Original languageEnglish (US)
Pages (from-to)5629-5643
Number of pages15
JournalBiomedical Optics Express
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2021

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Low-cost, chromatic confocal endomicroscope for cellular imaging in vivo'. Together they form a unique fingerprint.

Cite this