Abstract
We have previously reported that multiple injections of soluble MHC class I tetramers assembled with wild-type HY peptide induces unresponsiveness to male skin grafts in naive female C57BL/6 (B6) mice. Induction of unresponsiveness is dependent on a population of unresponsive allospecific CD8lo T cells. Reduced expression of CD8 acts to limit a T cell response to HY peptide by limiting the avidity window of effective signal transduction. We and others have demonstrated that CD8lo T cells are an alternative stable phenotype of CD8αβ+ T cells in vitro and in vivo after antigen stimulation. We show here that CD8lo T cells can suppress naive CD8+ T cell responses to HY antigen in vitro and male skin graft rejection in vivo after adoptive transfer into female recipients. These novel regulatory T cells express surface TGF-β1 and secrete T cytotoxic 2 cytokines after antigen-specific stimulation. Anti-TGF-β antibody and latency-associated peptide inhibit the suppressive effects in vitro. We also show that HY-specific memory CD8+ T cells overcome regulation by CD8lo T cells. These data define a novel peripheral regulatory CD8+ T cell population that arises after repeated antigen encounter in vivo. These cells have implications in the maintenance of tolerance and memory.
Original language | English (US) |
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Pages (from-to) | 397-410 |
Number of pages | 14 |
Journal | European Journal of Immunology |
Volume | 36 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2006 |
Keywords
- CD8 T cells
- Immune regulation
- MHC
- Regulatory T cells
- Tolerance Transplantation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology