Loss of acid ceramidase in myeloid cells suppresses intestinal neutrophil recruitment

Mel Pilar Espaillat, Ashley J. Snider, Zhijuan Qiu, Breana Channer, Nicolas Coant, Edward H. Schuchman, Richard R. Kew, Brian S. Sheridan, Yusuf A. Hannun, Lina M. Obeid

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Bioactive sphingolipids are modulators of immune processes and their metabolism is often dysregulated in ulcerative colitis, a major category of inflammatory bowel disease (IBD). While multiple axes of sphingolipid metabolism have been investigated to delineate mechanisms regulating ulcerative colitis, the role of acid ceramidase (AC) in intestinal inflammation is yet to be characterized. Here we demonstrate that AC expression is elevated selectively in the inflammatory infiltrate in human and murine colitis. To probe for mechanistic insight into how AC up-regulation can impact intestinal inflammation, we investigated the selective loss of AC expression in the myeloid population. Using a model of intestinal epithelial injury, we demonstrate that myeloid AC conditional knockout mice exhibit impairment of neutrophil recruitment to the colon mucosa as a result of defective cytokine and chemokine production. Furthermore, the loss of myeloid AC protects from tumor incidence in colitis-associated cancer (CAC) and inhibits the expansion of neutrophils and granulocytic myeloid-derived suppressor cells in the tumor microenvironment. Collectively, our results demonstrate a tissue-specific role for AC in regulating neutro-philic inflammation and cytokine production. We demonstrate novel mechanisms of how granulocytes are recruited to the colon that may have therapeutic potential in intestinal inflammation, IBD, and CAC.

Original languageEnglish (US)
Pages (from-to)2339-2353
Number of pages15
JournalFASEB Journal
Issue number5
StatePublished - May 2018
Externally publishedYes


  • Colitis-associated cancer
  • IBD
  • MDSC
  • Sphingolipids
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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