Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone

Kattika Kaarj, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Zika virus (ZIKV) infection may cause serious birth defects and is a critical concern for women of child-bearing age in affected regions. A simple, portable, and easy-to-use ZIKV detection method would enable point-of-care testing, which may aid in prevention of the spread of the virus. Herein, we describe a reverse transcription isothermal loop-mediated amplification (RT-LAMP) method that detects the presence of ZIKV RNA in complex samples (e.g., blood, urine, and tap water). Phenol red is the colorimetric indicator of successful amplification. Color changes based on the amplified RT-LAMP product from the presence of viral target are monitored using a smartphone camera under ambient light conditions. A single viral RNA molecule per μL can be detected in as quickly as 15 min using this method with 100% sensitivity and 100% specificity in blood and tap water, while 100% sensitivity and 67% specificity in urine. This platform can also be used to identify other viruses including SARS-CoV-2 and improve the current state of field-based diagnostics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages307-323
Number of pages17
DOIs
StatePublished - 2023
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2621
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bodily fluid
  • Colorimetry
  • RT-LAMP
  • Smartphone biosensor
  • ZIKV
  • Zika
  • μPAD

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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