Long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (UASB) bioreactor

Wenjie Sun; Reyes Sierra-Alvarez; Jim A. Field

doi:10.1016/j.biortech.2011.01.069

Long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (UASB) bioreactor

Wenjie Sun, Reyes Sierra-Alvarez, Jim A. Field

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A chlorate (ClO3-) reducing microbial consortium oxidized arsenite (As(III)) to arsenate (As(V)) in an upflow anaerobic sludge-bed bioreactor over 550days operation. As(III) was converted with high conversion efficiencies (>98%) at volumetric loadings ranging from 0.45 to 1.92mmol As/(L_reactord). The oxidation of As(III) was linked to the complete reduction of ClO3- to Cl^- and H₂O, as demonstrated by a molar ratio of approximately 3.0mol As(III) oxidized per mole of Cl^- formed and by the greatly lowered ClO3--reducing capacity without As(III) feeding. An autotrophic enrichment culture was established from the bioreactor biofilm. A 16S rRNA gene clone library indicated that the culture was dominated by Dechloromonas, and Stenotrophomonas as well as genera within the family Comamonadaceae. The results indicate that the oxidation of As(III) to less mobile As(V) utilizing ClO3- as a terminal electron acceptor provides a sustainable bioremediation strategy for arsenic contamination in anaerobic environments.

Original language	English (US)
Pages (from-to)	5010-5016
Number of pages	7
Journal	Bioresource Technology
Volume	102
Issue number	8
DOIs	https://doi.org/10.1016/j.biortech.2011.01.069
State	Published - Apr 2011

Keywords

Arsenite oxidation
Bioremediation
Chlorate reduction
Clone library
UASB bioreactor

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2011.01.069

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title = "Long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (UASB) bioreactor",

abstract = "A chlorate (ClO3-) reducing microbial consortium oxidized arsenite (As(III)) to arsenate (As(V)) in an upflow anaerobic sludge-bed bioreactor over 550days operation. As(III) was converted with high conversion efficiencies (>98%) at volumetric loadings ranging from 0.45 to 1.92mmol As/(Lreactord). The oxidation of As(III) was linked to the complete reduction of ClO3- to Cl- and H2O, as demonstrated by a molar ratio of approximately 3.0mol As(III) oxidized per mole of Cl- formed and by the greatly lowered ClO3--reducing capacity without As(III) feeding. An autotrophic enrichment culture was established from the bioreactor biofilm. A 16S rRNA gene clone library indicated that the culture was dominated by Dechloromonas, and Stenotrophomonas as well as genera within the family Comamonadaceae. The results indicate that the oxidation of As(III) to less mobile As(V) utilizing ClO3- as a terminal electron acceptor provides a sustainable bioremediation strategy for arsenic contamination in anaerobic environments.",

keywords = "Arsenite oxidation, Bioremediation, Chlorate reduction, Clone library, UASB bioreactor",

author = "Wenjie Sun and Reyes Sierra-Alvarez and Field, {Jim A.}",

note = "Funding Information: The work presented here was funded by a USGS, National Institute for Water Resources 104G Grant ( 2005AZ114G ), and by a grant of the NIEHS-supported Superfund Basic Research Program ( NIH ES-04940 ). The use of trade, product, or firm names in this report is for descriptive purposes only and does not constitute endorsement by the US Geological Survey. Some arsenic analyses were performed by the Analytical Section of the Hazard Identification Core ( Superfund Basic Research Program Grant NIEHS-04940 ). ",

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AU - Sierra-Alvarez, Reyes

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N1 - Funding Information: The work presented here was funded by a USGS, National Institute for Water Resources 104G Grant ( 2005AZ114G ), and by a grant of the NIEHS-supported Superfund Basic Research Program ( NIH ES-04940 ). The use of trade, product, or firm names in this report is for descriptive purposes only and does not constitute endorsement by the US Geological Survey. Some arsenic analyses were performed by the Analytical Section of the Hazard Identification Core ( Superfund Basic Research Program Grant NIEHS-04940 ).

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N2 - A chlorate (ClO3-) reducing microbial consortium oxidized arsenite (As(III)) to arsenate (As(V)) in an upflow anaerobic sludge-bed bioreactor over 550days operation. As(III) was converted with high conversion efficiencies (>98%) at volumetric loadings ranging from 0.45 to 1.92mmol As/(Lreactord). The oxidation of As(III) was linked to the complete reduction of ClO3- to Cl- and H2O, as demonstrated by a molar ratio of approximately 3.0mol As(III) oxidized per mole of Cl- formed and by the greatly lowered ClO3--reducing capacity without As(III) feeding. An autotrophic enrichment culture was established from the bioreactor biofilm. A 16S rRNA gene clone library indicated that the culture was dominated by Dechloromonas, and Stenotrophomonas as well as genera within the family Comamonadaceae. The results indicate that the oxidation of As(III) to less mobile As(V) utilizing ClO3- as a terminal electron acceptor provides a sustainable bioremediation strategy for arsenic contamination in anaerobic environments.

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Long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (UASB) bioreactor

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