@inbook{532f7dcb7d324822b73d3e8c0d2259bf,
title = "Long-term culture of nephron progenitor cells ex vivo",
abstract = "Nephrons differentiate from the cap mesenchyme of the fetal kidney. Nephron progenitor cells that populate the cap mesenchyme efficiently balance self-renewal and epithelial differentiation to enable repeated rounds of nephron formation during development. Here we describe a method to isolate and propagate these cells from the embryonic mouse kidney. Using this method, nephron progenitor cells from a single litter of mice can be propagated to hundreds of millions of cells that express appropriate markers of the undifferentiated state and retain epithelial differentiation capacity in vitro.",
keywords = "Kidney, Organogenesis, Primary cell culture, Stem cells",
author = "Brown, {Aaron C.} and Gupta, {Ashwani K.} and Leif Oxburgh",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9021-4_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--75",
booktitle = "Methods in Molecular Biology",
}