@article{f23227562b1b46d8a8a356e7760b20d6,
title = "Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma",
abstract = "Researchers develop and test an nitric oxide (NO) delivery platform that directly targets the conventional outflow pathway and locally liberates a controlled dose of NO via enzyme biocatalysis. Enzymes are embedded at the desired sites and serve as biological machinery that can locally convert externally administered NO donors into active therapeutics. To enmesh enzymes deep within the TM, which is the principal resistance-generating region, the researchers encapsulate Β-galactosidase in polymer carriers. They fabricate polymer carrier capsules via layer-by-layer adsorption of interacting polymers onto sacrificial particle templates, a versatile technique that allows incorporation of an extensive choice of materials within the multilayer structures and gives fine control over the diffusion of molecules across the shell of the polymer capsules.",
keywords = "glaucoma, layer-by-layer capsules, liposomes, nitric oxide, β-galactosidase",
author = "Rona Chandrawati and Chang, {Jason Y.H.} and Ester Reina-Torres and Coline Jumeaux and Sherwood, {Joseph M.} and Stamer, {W. Daniel} and Zelikin, {Alexander N.} and Overby, {Darryl R.} and Stevens, {Molly M.}",
note = "Funding Information: R.C. and J.Y.H.C. contributed equally to this work. The authors thank Jacques Bertrand and Anabela Cepa Areias (Imperial College London) for their technical assistance, and Dr. Cristoforo Silvestri (Imperial College London) for critical discussion of the data. R.C. acknowledges the support from the Marie Curie International Incoming Fellowship within the 7th Framework Programme under Grant Agreement No. 299936. J.Y.H.C acknowledges the support from the PhD Studentship from Department of Bioengineering, Imperial College London. W.D.S. and D.R.O. acknowledge funding from Fight for Sight (UK), the National Glaucoma Research Program of the BrightFocus Foundation, and the National Eye Institute (EY022359). A.N.Z. acknowledges funding from the European Research Council Consolidator Grant (ERC-2013-CoG 617336 BTVI). C.J. and M.M.S. gratefully acknowledge financial support from the Rosetrees Trust. M.M.S. acknowledges the support from the ERC Seventh Framework Programme Consolidator grant “Naturale CG” under Grant Agreement No. 616417, the Engineering and Physical Sciences Research Council (EPSRC) grant “Bio-functionalised nanomaterials for ultrasensitive biosensing” (EP/K020641/1), and a Wellcome Trust Senior Investigator Award (098411/Z/12/Z). The raw data supporting this publication is available online at https://doi.org/10.5281/zenodo.223934.",
year = "2017",
month = apr,
day = "25",
doi = "10.1002/adma.201604932",
language = "English (US)",
volume = "29",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-Blackwell",
number = "16",
}