Localization of zip14 and zip8 in hibcpp cells

Shannon E. Morgan, Horst Schroten, Hiroshi Ishikawa, Ningning Zhao

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The blood–cerebrospinal fluid barrier (BCB) is important in maintaining brain manganese (Mn) homeostasis. This barrier consists of a single layer of epithelial cells, connected by tight junctions, that restrict the passage of nutrients to only allow molecules to be carried through the membrane by a transporter. These epithelial cells are polarized with asymmetrical blood-facing and cerebrospinal fluid-facing sides. Here, we have established a polarized model of a human choroid plexus papilloma cell line, HIBCPP. For the first time, Mn importers ZIP14 and ZIP8 were identified in HIBCPP cells and were found to be enriched at the basolateral and apical sides of the cell monolayer, respectively. The localization of each ZIP protein adds to the understanding of Mn transport across the HIBCPP BCB model to help understand the mechanism of Mn homeostasis within the brain.

Original languageEnglish (US)
Article number534
Pages (from-to)1-15
Number of pages15
JournalBrain Sciences
Volume10
Issue number8
DOIs
StatePublished - Aug 2020

Keywords

  • Blood-cerebrospinal fluid barrier
  • HIBCPP
  • Manganese
  • SLC39A14
  • SLC39A8
  • ZIP14
  • ZIP8

ASJC Scopus subject areas

  • Neuroscience(all)

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