Localization of zip14 and zip8 in hibcpp cells

Shannon E. Morgan; Horst Schroten; Hiroshi Ishikawa; Ningning Zhao

doi:10.3390/brainsci10080534

Localization of zip14 and zip8 in hibcpp cells

Shannon E. Morgan, Horst Schroten, Hiroshi Ishikawa, Ningning Zhao

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The blood–cerebrospinal fluid barrier (BCB) is important in maintaining brain manganese (Mn) homeostasis. This barrier consists of a single layer of epithelial cells, connected by tight junctions, that restrict the passage of nutrients to only allow molecules to be carried through the membrane by a transporter. These epithelial cells are polarized with asymmetrical blood-facing and cerebrospinal fluid-facing sides. Here, we have established a polarized model of a human choroid plexus papilloma cell line, HIBCPP. For the first time, Mn importers ZIP14 and ZIP8 were identified in HIBCPP cells and were found to be enriched at the basolateral and apical sides of the cell monolayer, respectively. The localization of each ZIP protein adds to the understanding of Mn transport across the HIBCPP BCB model to help understand the mechanism of Mn homeostasis within the brain.

Original language	English (US)
Article number	534
Pages (from-to)	1-15
Number of pages	15
Journal	Brain Sciences
Volume	10
Issue number	8
DOIs	https://doi.org/10.3390/brainsci10080534
State	Published - Aug 2020

Keywords

Blood-cerebrospinal fluid barrier
HIBCPP
Manganese
SLC39A14
SLC39A8
ZIP14
ZIP8

ASJC Scopus subject areas

General Neuroscience

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10.3390/brainsci10080534

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title = "Localization of zip14 and zip8 in hibcpp cells",

abstract = "The blood–cerebrospinal fluid barrier (BCB) is important in maintaining brain manganese (Mn) homeostasis. This barrier consists of a single layer of epithelial cells, connected by tight junctions, that restrict the passage of nutrients to only allow molecules to be carried through the membrane by a transporter. These epithelial cells are polarized with asymmetrical blood-facing and cerebrospinal fluid-facing sides. Here, we have established a polarized model of a human choroid plexus papilloma cell line, HIBCPP. For the first time, Mn importers ZIP14 and ZIP8 were identified in HIBCPP cells and were found to be enriched at the basolateral and apical sides of the cell monolayer, respectively. The localization of each ZIP protein adds to the understanding of Mn transport across the HIBCPP BCB model to help understand the mechanism of Mn homeostasis within the brain.",

keywords = "Blood-cerebrospinal fluid barrier, HIBCPP, Manganese, SLC39A14, SLC39A8, ZIP14, ZIP8",

author = "Morgan, {Shannon E.} and Horst Schroten and Hiroshi Ishikawa and Ningning Zhao",

note = "Funding Information: Funding: This work was supported by the National Institutes of Health Grants R00DK104066 (to N.Z.). Publisher Copyright: {\textcopyright} 2020 by the authors.",

year = "2020",

month = aug,

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language = "English (US)",

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AU - Morgan, Shannon E.

AU - Schroten, Horst

AU - Ishikawa, Hiroshi

AU - Zhao, Ningning

PY - 2020/8

Y1 - 2020/8

N2 - The blood–cerebrospinal fluid barrier (BCB) is important in maintaining brain manganese (Mn) homeostasis. This barrier consists of a single layer of epithelial cells, connected by tight junctions, that restrict the passage of nutrients to only allow molecules to be carried through the membrane by a transporter. These epithelial cells are polarized with asymmetrical blood-facing and cerebrospinal fluid-facing sides. Here, we have established a polarized model of a human choroid plexus papilloma cell line, HIBCPP. For the first time, Mn importers ZIP14 and ZIP8 were identified in HIBCPP cells and were found to be enriched at the basolateral and apical sides of the cell monolayer, respectively. The localization of each ZIP protein adds to the understanding of Mn transport across the HIBCPP BCB model to help understand the mechanism of Mn homeostasis within the brain.

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KW - Blood-cerebrospinal fluid barrier

KW - HIBCPP

KW - Manganese

KW - SLC39A14

KW - SLC39A8

KW - ZIP14

KW - ZIP8

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Localization of zip14 and zip8 in hibcpp cells

Abstract

Keywords

ASJC Scopus subject areas

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