Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex

Rose S. Yamazaki; Qi Chen; Steven S. Schreiber; Roberta Diaz Brinton

doi:10.1016/S0169-328X(96)00285-9

Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex

Rose S. Yamazaki, Qi Chen, Steven S. Schreiber, Roberta Diaz Brinton

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Previous studies suggested the existence of V1a vasopressin receptors (V1aR) in the cerebral cortex. Here, we investigated the cellular and regional localization of V1aR in the E18 rat cerebral cortex using RT-PCR and Southern blot analysis of V1aR mRNA derived from enriched cultures of neurons, astroglia, and oligodendroglia from four cortical regions (rostral, caudal, dorsal and ventral). V1aR mRNA was detected in each of the cell types within each of the regions studied. These data indicate that V1aR is broadly distributed throughout the cerebral cortex and suggest that vasopressin plays an important role in cortical functions.

Original language	English (US)
Pages (from-to)	138-140
Number of pages	3
Journal	Molecular Brain Research
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/S0169-328X(96)00285-9
State	Published - Apr 1997
Externally published	Yes

Keywords

Astroglia
Cerebral cortex
Learning and memory
Neuron
Oligodendroglia
RT-PCR
Vasopressin receptor
mRNA

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(96)00285-9

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title = "Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex",

abstract = "Previous studies suggested the existence of V1a vasopressin receptors (V1aR) in the cerebral cortex. Here, we investigated the cellular and regional localization of V1aR in the E18 rat cerebral cortex using RT-PCR and Southern blot analysis of V1aR mRNA derived from enriched cultures of neurons, astroglia, and oligodendroglia from four cortical regions (rostral, caudal, dorsal and ventral). V1aR mRNA was detected in each of the cell types within each of the regions studied. These data indicate that V1aR is broadly distributed throughout the cerebral cortex and suggest that vasopressin plays an important role in cortical functions.",

keywords = "Astroglia, Cerebral cortex, Learning and memory, Neuron, Oligodendroglia, RT-PCR, Vasopressin receptor, mRNA",

author = "Yamazaki, {Rose S.} and Qi Chen and Schreiber, {Steven S.} and Brinton, {Roberta Diaz}",

note = "Funding Information: Research was supported by NSF Grant IBN-95 11423 (to R.D.B.) and by a AFPE-Bristol-Meyers Squibb Fellowship (to R.S.Y.). We thank Dr. S.J. Lolait for providing the plasmids, Dr. W. Gilmore for expert guidance and assistance in selective glial culturing techniques, and Ning Sun for her expert assistance in RT-PCR.",

year = "1997",

month = apr,

doi = "10.1016/S0169-328X(96)00285-9",

language = "English (US)",

volume = "45",

pages = "138--140",

journal = "Molecular Brain Research",

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T1 - Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex

AU - Yamazaki, Rose S.

AU - Chen, Qi

AU - Schreiber, Steven S.

AU - Brinton, Roberta Diaz

N1 - Funding Information: Research was supported by NSF Grant IBN-95 11423 (to R.D.B.) and by a AFPE-Bristol-Meyers Squibb Fellowship (to R.S.Y.). We thank Dr. S.J. Lolait for providing the plasmids, Dr. W. Gilmore for expert guidance and assistance in selective glial culturing techniques, and Ning Sun for her expert assistance in RT-PCR.

PY - 1997/4

Y1 - 1997/4

N2 - Previous studies suggested the existence of V1a vasopressin receptors (V1aR) in the cerebral cortex. Here, we investigated the cellular and regional localization of V1aR in the E18 rat cerebral cortex using RT-PCR and Southern blot analysis of V1aR mRNA derived from enriched cultures of neurons, astroglia, and oligodendroglia from four cortical regions (rostral, caudal, dorsal and ventral). V1aR mRNA was detected in each of the cell types within each of the regions studied. These data indicate that V1aR is broadly distributed throughout the cerebral cortex and suggest that vasopressin plays an important role in cortical functions.

AB - Previous studies suggested the existence of V1a vasopressin receptors (V1aR) in the cerebral cortex. Here, we investigated the cellular and regional localization of V1aR in the E18 rat cerebral cortex using RT-PCR and Southern blot analysis of V1aR mRNA derived from enriched cultures of neurons, astroglia, and oligodendroglia from four cortical regions (rostral, caudal, dorsal and ventral). V1aR mRNA was detected in each of the cell types within each of the regions studied. These data indicate that V1aR is broadly distributed throughout the cerebral cortex and suggest that vasopressin plays an important role in cortical functions.

KW - Astroglia

KW - Cerebral cortex

KW - Learning and memory

KW - Neuron

KW - Oligodendroglia

KW - RT-PCR

KW - Vasopressin receptor

KW - mRNA

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DO - 10.1016/S0169-328X(96)00285-9

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AN - SCOPUS:0031127889

SN - 0169-328X

VL - 45

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JO - Molecular Brain Research

JF - Molecular Brain Research

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Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex

Abstract

Keywords

ASJC Scopus subject areas

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