Localization of PEPT1 and PEPT2 proton-coupled oligopeptide transporter mRNA and protein in rat kidney

Hong Shen, David E. Smith, Tianxin Yang, Yuning G. Huang, Jürgen B. Schneemann, Frank C. Brosius

Research output: Contribution to journalArticlepeer-review

183 Scopus citations

Abstract

To determine the renal localization of oligopeptide transporters, Northern blot analyses were performed and polyclonal antisera were generated against PEPT1 and PEPT2, the two cloned rat H+/peptide transporters. Under high-stringency conditions, a 3.0-kb mRNA transcript of rat PEPT1 was expressed primarily in superficial cortex, whereas a 3.5-kb mRNA transcript of PEPT2 was expressed primarily in deep cortex/ outer stripe of outer medulla. PEPT1 antisera detected a specific band on immunoblots of renal and intestinal brush-border membrane vesicles (BBMV) with an apparent mobility of ~90 kDa. PEPT2 antisera detected a specific broad band of ~85 kDa in renal but not in intestinal BBMV. PEPT1 immunolocalization experiments showed detection of a brush border antigen in S1 segments of the proximal tubule and in the brush border of villi from all segments of the small intestine. In contrast, PEPT2 immunolocalization was primarily confined to the brush border of S3 segments of the proximal tubule. All other nephron segments in rat were negative for PEPT1 and PEPT2 staining. Overall, our results conclusively demonstrate that although PEPT1 is expressed in early regions of the proximal tubule (pars convoluta), PEPT2 is specific for the latter regions of proximal tubule (pars recta).

Original languageEnglish (US)
Pages (from-to)F658-F665
JournalAmerican Journal of Physiology - Renal Physiology
Volume276
Issue number5 45-5
DOIs
StatePublished - May 1999
Externally publishedYes

Keywords

  • Immunoblots
  • Immunocytochemistry
  • Northern blot analysis
  • Proximal tubules

ASJC Scopus subject areas

  • Physiology
  • Urology

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