TY - JOUR
T1 - Localization of cells containing estrogen receptor-like immunoreactivity in the Brazilian opossum brain
AU - Fox, Charles A.
AU - Ross, Lynne R.
AU - Handa, Robert J.
AU - Jacobson, Carol D.
N1 - Funding Information:
nical assistance, and Cathy Martens for photographic assistance. We also thank the Iowa State University Image Analysis facility for providing the Zeiss Axiophot microscope. The authors also wish to acknowledge Dr. Joan Morrell for several helpful discussions. This work was supported by NSF Grant BNS 8909751U, SDA formula funds, and an Iowa State University research minigrant.
PY - 1991/4/12
Y1 - 1991/4/12
N2 - The Brazilian opossum (Monodelphis domestica) is a small, pouchless marsupial whose young are born in an immature, sexually undifferentiated state. Etgen and Fadem10, and Handa and coworkers13 have biochemically detected and characterized estrogen receptors in the forebrain of the Brazilian opossum. In this study, we have examine the distribution of estrogen receptor-like immunoreactive (ER-LI) cells in the brains of gonadectomized male and female Brazilian opossums using Abbott H222 rat monoclonal estrogen receptor antibody (H222 is a gift of Abbott Labs). An indirect immunohistochemical procedure employing the Vectastain Elite system and a nickel-enhanced DAB chromogen was used. A large number of ER-LI cell nuclei were observed in the medial preoptic area, ventral septal nucleus, medial division of the bed nucleus of the stria terminalis, lateral part of the ventromedial hypothalamus, premammillary nucleus, arcuate nucleus, posterior amygdaloid nucleus, and the midbrain central grey. Lower numbers of ER-LI cell nuclei were observed in the intermediate subdivision of the lateral sepatal nucleus, and in the anterior, medial, and posterior cortical amygdloid nuclei. The anatomical distribution of ER-LI in the Brazilian opossum brain is similar to that which has been reported for estrogen binding sites following biochemical analysis. Based on these findings, we believe specific regions of the Brazilian opossum brain may serve as substrata for the action of estrogen in the adult. In addition, these results are supportive of the use of this animal model to investigate the organizational effects of estrogen on the developing central nervous system.
AB - The Brazilian opossum (Monodelphis domestica) is a small, pouchless marsupial whose young are born in an immature, sexually undifferentiated state. Etgen and Fadem10, and Handa and coworkers13 have biochemically detected and characterized estrogen receptors in the forebrain of the Brazilian opossum. In this study, we have examine the distribution of estrogen receptor-like immunoreactive (ER-LI) cells in the brains of gonadectomized male and female Brazilian opossums using Abbott H222 rat monoclonal estrogen receptor antibody (H222 is a gift of Abbott Labs). An indirect immunohistochemical procedure employing the Vectastain Elite system and a nickel-enhanced DAB chromogen was used. A large number of ER-LI cell nuclei were observed in the medial preoptic area, ventral septal nucleus, medial division of the bed nucleus of the stria terminalis, lateral part of the ventromedial hypothalamus, premammillary nucleus, arcuate nucleus, posterior amygdaloid nucleus, and the midbrain central grey. Lower numbers of ER-LI cell nuclei were observed in the intermediate subdivision of the lateral sepatal nucleus, and in the anterior, medial, and posterior cortical amygdloid nuclei. The anatomical distribution of ER-LI in the Brazilian opossum brain is similar to that which has been reported for estrogen binding sites following biochemical analysis. Based on these findings, we believe specific regions of the Brazilian opossum brain may serve as substrata for the action of estrogen in the adult. In addition, these results are supportive of the use of this animal model to investigate the organizational effects of estrogen on the developing central nervous system.
KW - Estrogen receptor
KW - Marsupial
KW - Medial preoptic area
KW - Steroid receptor
KW - Ventromedial hypothalamus
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U2 - 10.1016/0006-8993(91)91163-U
DO - 10.1016/0006-8993(91)91163-U
M3 - Article
C2 - 1855151
AN - SCOPUS:0025752613
SN - 0006-8993
VL - 546
SP - 96
EP - 105
JO - Brain Research
JF - Brain Research
IS - 1
ER -