Local MicroRNA modulation using a novel Anti-MIR-21-eluting stent effectively prevents experimental in-stent restenosis

Dong Wang, Tobias Deuse, Mandy Stubbendorff, Ekaterina Chernogubova, Reinhold G. Erben, Suzanne M. Eken, Hong Jin, Yuhuang Li, Albert Busch, Christian H. Heeger, Boris Behnisch, Hermann Reichenspurner, Robert C. Robbins, Joshua M. Spin, Philip S. Tsao, Sonja Schrepfer, Lars Maegdefessel

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

Objective - Despite advances in stent technology for vascular interventions, in-stent restenosis (ISR) because of myointimal hyperplasia remains a major complication. Approach and Results - We investigated the regulatory role of microRNAs in myointimal hyperplasia/ISR, using a humanized animal model in which balloon-injured human internal mammary arteries with or without stenting were transplanted into Rowett nude rats, followed by microRNA profiling. miR-21 was the only significantly upregulated candidate. In addition, miR-21 expression was increased in human tissue samples from patients with ISR compared with coronary artery disease specimen. We systemically repressed miR-21 via intravenous fluorescein-tagged-locked nucleic acid-anti-miR-21 (anti-21) in our humanized myointimal hyperplasia model. As expected, suppression of vascular miR-21 correlated dose dependently with reduced luminal obliteration. Furthermore, anti-21 did not impede reendothelialization. However, systemic anti-miR-21 had substantial off-target effects, lowering miR-21 expression in liver, heart, lung, and kidney with concomitant increase in serum creatinine levels. We therefore assessed the feasibility of local miR-21 suppression using anti-21-coated stents. Compared with bare-metal stents, anti-21-coated stents effectively reduced ISR, whereas no significant off-target effects could be observed. Conclusion - This study demonstrates the efficacy of an anti-miR-coated stent for the reduction of ISR.

Original languageEnglish (US)
Pages (from-to)1945-1953
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume35
Issue number9
DOIs
StatePublished - Sep 28 2015
Externally publishedYes

Keywords

  • coronary restenosis
  • hyperplasia
  • microRNAs
  • rats
  • stents

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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