Local conformational changes of vitronectin upon adsorption on glass and silane surfaces

Filip Banovac, Steven S. Saavedra, George A. Truskey

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The conformation of vitronectin (Vn) in solution and adsorbed to hydrophilic and hydrophobic glass was studied using the polarity-sensitive fluor acrylodan. Free sulfhydryl groups of vitronectin were labeled with acrylodan under nondenaturing conditions. The number and location of the free sulfhydryl groups were determined by cleavage with either formic acid or cyanogen bromide followed by polyacrylamide gel electrophoresis. Based upon published amino acid sequences of Vn, the fluorescence of acrylodan-labeled Vn (Ac-Vn) fragments is consistent with free sulfhydryls being located at cysteine residues 196 and 274. Conformational changes upon adsorption on glass and silanized glass surfaces placed the free sulfhydryl residues in a more hydrophobic environment than was observed when Vn was in PBS solution. Small but statistically significant quenching of Ac-Vn fluorescence with iodide could be explained in terms of a two-site model. Results of this study suggest that the domains surrounding the free sulfhydryl groups of vitronectin experience different environments in solution and adsorbed to surfaces.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalJournal of Colloid And Interface Science
Issue number1
StatePublished - Jun 1994

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry


Dive into the research topics of 'Local conformational changes of vitronectin upon adsorption on glass and silane surfaces'. Together they form a unique fingerprint.

Cite this