Liver lesions produced by aflatoxins in Rana catesbeiana (bullfrog)

Tony Fernando Grassi, Paulo Wagner Pires, Luis Fernando Barbisan, Maeli Dal Pai-Silva, Roueda Abou Said, João Lauro Viana de Camargo

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


This study describes alterations induced in Rana catesbeiana (bullfrog) liver after extended dietary exposure to aflatoxins (AFs). Bullfrogs of both sexes were fed for 120 days a commercial chow blended with a rice bran-based mixture of AFs containing 667.0, 11.65, 141.74, and 3.53 mg/kg of AFs B1, B2, G1, and G2, respectively. Animals were sacrificed on study days 45, 90, and 120. Severe and progressive liver lesions with structural collapse, increased hepatocyte and biliary duct cell proliferation, appearance of basophilic hepatocytes, and diffuse scarring, were observed at all time points. There were no quantitative alterations in the liver melanomacrophage centers of the AFs-exposed animals. Increased amounts of lipid hydroperoxides, indicative of ongoing oxidative stress, were more evident in the Addutor magnum muscle than in the AFs-damaged livers. No tumors were found in the R. catesbeiana livers after 120 days of exposure to relatively high doses of AFs.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalEcotoxicology and Environmental Safety
Issue number1
StatePublished - Sep 2007
Externally publishedYes


  • Aflatoxins
  • Bullfrogs
  • Liver toxicity
  • Rana catesbeiana

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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