TY - JOUR
T1 - Liver lesions produced by aflatoxins in Rana catesbeiana (bullfrog)
AU - Grassi, Tony Fernando
AU - Pires, Paulo Wagner
AU - Barbisan, Luis Fernando
AU - Pai-Silva, Maeli Dal
AU - Said, Roueda Abou
AU - de Camargo, João Lauro Viana
N1 - Funding Information:
Ethel Lourenzi Barbosa Novelli (Instituto de Biociências, UNESP, Botucatu, Brazil) and Maria Antonia Calori Domingues (ESALQ, USP, Piracicaba, Brazil) performed lipid hydroperoxide determinations in the bullfrog tissues and aflatoxin levels determinations in the diets, respectively. Lora Arnold (University of Nebraska Medical Center, USA) kindly reviewed the manuscript and gave invaluable suggestion. This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Grant # 99/07059-1). TFG and PWP were recipients of scholarships from FAPESP (#99/10540-3) and CNPq (PIBIC/UNESP), respectively. JLVC is a CNPq Research Fellow (#302361/2003-0). The experimental protocol of this study was approved by the UNESP Medical School Committee for Ethics in Animal Research (Protocol # 103/02).
PY - 2007/9
Y1 - 2007/9
N2 - This study describes alterations induced in Rana catesbeiana (bullfrog) liver after extended dietary exposure to aflatoxins (AFs). Bullfrogs of both sexes were fed for 120 days a commercial chow blended with a rice bran-based mixture of AFs containing 667.0, 11.65, 141.74, and 3.53 mg/kg of AFs B1, B2, G1, and G2, respectively. Animals were sacrificed on study days 45, 90, and 120. Severe and progressive liver lesions with structural collapse, increased hepatocyte and biliary duct cell proliferation, appearance of basophilic hepatocytes, and diffuse scarring, were observed at all time points. There were no quantitative alterations in the liver melanomacrophage centers of the AFs-exposed animals. Increased amounts of lipid hydroperoxides, indicative of ongoing oxidative stress, were more evident in the Addutor magnum muscle than in the AFs-damaged livers. No tumors were found in the R. catesbeiana livers after 120 days of exposure to relatively high doses of AFs.
AB - This study describes alterations induced in Rana catesbeiana (bullfrog) liver after extended dietary exposure to aflatoxins (AFs). Bullfrogs of both sexes were fed for 120 days a commercial chow blended with a rice bran-based mixture of AFs containing 667.0, 11.65, 141.74, and 3.53 mg/kg of AFs B1, B2, G1, and G2, respectively. Animals were sacrificed on study days 45, 90, and 120. Severe and progressive liver lesions with structural collapse, increased hepatocyte and biliary duct cell proliferation, appearance of basophilic hepatocytes, and diffuse scarring, were observed at all time points. There were no quantitative alterations in the liver melanomacrophage centers of the AFs-exposed animals. Increased amounts of lipid hydroperoxides, indicative of ongoing oxidative stress, were more evident in the Addutor magnum muscle than in the AFs-damaged livers. No tumors were found in the R. catesbeiana livers after 120 days of exposure to relatively high doses of AFs.
KW - Aflatoxins
KW - Bullfrogs
KW - Liver toxicity
KW - Rana catesbeiana
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U2 - 10.1016/j.ecoenv.2006.07.007
DO - 10.1016/j.ecoenv.2006.07.007
M3 - Article
C2 - 16996133
AN - SCOPUS:34447572957
SN - 0147-6513
VL - 68
SP - 71
EP - 78
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
IS - 1
ER -