Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Ruslan Rafikov; Christiana Dimitropoulou; Saurabh Aggarwal; Archana Kangath; Christine Gross; Daniel Pardo; Shruti Sharma; Agnieszka Jezierska-Drutel; Vijay Patel; Connie Snead; Rudolf Lucas; Alexander Verin; David Fulton; John D. Catravas; Stephen M. Black

doi:10.1074/jbc.M114.547596

Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Ruslan Rafikov
, Christiana Dimitropoulou
, Saurabh Aggarwal
, Archana Kangath
, Christine Gross
, Daniel Pardo
, Shruti Sharma
, Agnieszka Jezierska-Drutel
, Vijay Patel
, Connie Snead
, Rudolf Lucas
, Alexander Verin
, David Fulton
, John D. Catravas
, Stephen M. Black

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr³⁴ produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.

Original language	English (US)
Pages (from-to)	4710-4722
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	289
Issue number	8
DOIs	https://doi.org/10.1074/jbc.M114.547596
State	Published - Feb 21 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Rafikov, R., Dimitropoulou, C., Aggarwal, S., Kangath, A., Gross, C., Pardo, D., Sharma, S., Jezierska-Drutel, A., Patel, V., Snead, C., Lucas, R., Verin, A., Fulton, D., Catravas, J. D., & Black, S. M. (2014). Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA. Journal of Biological Chemistry, 289(8), 4710-4722. https://doi.org/10.1074/jbc.M114.547596

Rafikov, R, Dimitropoulou, C, Aggarwal, S, Kangath, A, Gross, C, Pardo, D, Sharma, S, Jezierska-Drutel, A, Patel, V, Snead, C, Lucas, R, Verin, A, Fulton, D, Catravas, JD & Black, SM 2014, 'Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA', Journal of Biological Chemistry, vol. 289, no. 8, pp. 4710-4722. https://doi.org/10.1074/jbc.M114.547596

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title = "Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA",

abstract = "Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr34 produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.",

author = "Ruslan Rafikov and Christiana Dimitropoulou and Saurabh Aggarwal and Archana Kangath and Christine Gross and Daniel Pardo and Shruti Sharma and Agnieszka Jezierska-Drutel and Vijay Patel and Connie Snead and Rudolf Lucas and Alexander Verin and David Fulton and Catravas, \{John D.\} and Black, \{Stephen M.\}",

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doi = "10.1074/jbc.M114.547596",

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AU - Rafikov, Ruslan

AU - Dimitropoulou, Christiana

AU - Aggarwal, Saurabh

AU - Kangath, Archana

AU - Gross, Christine

AU - Pardo, Daniel

AU - Sharma, Shruti

AU - Jezierska-Drutel, Agnieszka

AU - Patel, Vijay

AU - Snead, Connie

AU - Lucas, Rudolf

AU - Verin, Alexander

AU - Fulton, David

AU - Catravas, John D.

AU - Black, Stephen M.

PY - 2014/2/21

Y1 - 2014/2/21

N2 - Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr34 produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.

AB - Background: The activation of RhoA is a critical event in acute lung injury (ALI), but the role of nitration in this process is unresolved. Results: The nitration of RhoA at Tyr34 produced GEF-like conformational changes that stimulate RhoA by decreasing GDP binding. Conclusion: We have identified a new mechanism of RhoA activation. Significance: Preventing RhoA nitration may be useful for the management of ALI.

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EP - 4722

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

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Lipopolysaccharide-induced lung injury involves the nitration-mediated activation of RhoA

Abstract

ASJC Scopus subject areas

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