Lipid bilayer dynamics and rhodopsin-lipid interactions: New approach using high-resolution solid-state 13C NMR

Michael D. Sefcik; Jacob Schaefer; Edward O. Stejskal; Robert A. McKay; Jeffrey F. Ellena; Steven W. Dodd; Michael F. Brown

doi:10.1016/0006-291X(83)90668-X

Lipid bilayer dynamics and rhodopsin-lipid interactions: New approach using high-resolution solid-state ¹³C NMR

Michael D. Sefcik, Jacob Schaefer, Edward O. Stejskal, Robert A. McKay, Jeffrey F. Ellena, Steven W. Dodd, Michael F. Brown

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

High-resolution, solid-state ¹³C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of ¹H dipolar decoupling, ¹H-¹³C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame ¹³C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.

Original language	English (US)
Pages (from-to)	1048-1055
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	114
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)90668-X
State	Published - Aug 12 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Lipid bilayer dynamics and rhodopsin-lipid interactions: New approach using high-resolution solid-state 13C NMR",

abstract = "High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.",

author = "Sefcik, \{Michael D.\} and Jacob Schaefer and Stejskal, \{Edward O.\} and McKay, \{Robert A.\} and Ellena, \{Jeffrey F.\} and Dodd, \{Steven W.\} and Brown, \{Michael F.\}",

note = "Funding Information: NIH Grant (J.F.E.), Williams",

year = "1983",

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doi = "10.1016/0006-291X(83)90668-X",

language = "English (US)",

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T1 - Lipid bilayer dynamics and rhodopsin-lipid interactions

T2 - New approach using high-resolution solid-state 13C NMR

AU - Sefcik, Michael D.

AU - Schaefer, Jacob

AU - Stejskal, Edward O.

AU - McKay, Robert A.

AU - Ellena, Jeffrey F.

AU - Dodd, Steven W.

AU - Brown, Michael F.

N1 - Funding Information: NIH Grant (J.F.E.), Williams

PY - 1983/8/12

Y1 - 1983/8/12

N2 - High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.

AB - High-resolution, solid-state 13C NMR spectra have been obtained for unsonicated multilamellar dispersions of 1,2-dilauryl-sn-glycero-3-phosphocholine (DLPC), recombinant membranes containing DLPC and rhodopsin, and native retinal rod disk membranes. The roles of 1H dipolar decoupling, 1H-13C cross-polarization, and magic-angle sample spinning have been investigated. Rotating-frame 13C relaxation times have been measured and are discussed in terms of lipid bilayer dynamics and rhodopsin-lipid interactions.

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Lipid bilayer dynamics and rhodopsin-lipid interactions: New approach using high-resolution solid-state ¹³C NMR

Abstract

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