TY - JOUR
T1 - Light-triggered and cysteine-mediated nitric oxide release from a biodegradable starch-based film
AU - Roveda, Antonio Carlos
AU - De Fazio Aguiar, Helena
AU - Miranda, Katrina M.
AU - Tadini, Carmen Cecília
AU - Franco, Douglas Wagner
N1 - Publisher Copyright:
© 2014 the Partner Organisations.
PY - 2014/11/7
Y1 - 2014/11/7
N2 - A new nitric oxide-releasing material produced with cassava starch is described. The ruthenium nitrosyl complex trans-[Ru(NH3)4(isn)NO](BF4)3 (RuNOisn; isn = isonicotinamide) is able to release NO upon either photolysis or chemical reduction. Impregnating this complex under mild conditions into cassava starch (CS) films produced a NO-delivery platform (CSx-RuNOisn). Spectroscopic analysis of CSx-RuNOisn indicates that the coordination sphere of RuNOisn remains intact during film production. Exposure of CSx-RuNOisn to long wave UV-light (λirr = 355 nm) leads to NO release and formation of the paramagnetic photoproduct trans-[RuIII(NH3)4isn(H2O)]3+ in the CS film. Reaction of this aquaruthenium(iii) complex with aqueous nitrite regenerates RuNOisn in the film. Delivery of NO upon photolysis of CSx-RuNOisn was verified by trapping with oxymyoglobin. Moreover, NO release upon chemical reduction was carried out using l-cysteine as a reductant. Cysteine-mediated NO delivery from CSx-RuNOisn persisted for more than 7 h, during which physiologically relevant NO concentrations were liberated. These results suggest that CSx-RuNOisn is a promising candidate for use in biological applications.
AB - A new nitric oxide-releasing material produced with cassava starch is described. The ruthenium nitrosyl complex trans-[Ru(NH3)4(isn)NO](BF4)3 (RuNOisn; isn = isonicotinamide) is able to release NO upon either photolysis or chemical reduction. Impregnating this complex under mild conditions into cassava starch (CS) films produced a NO-delivery platform (CSx-RuNOisn). Spectroscopic analysis of CSx-RuNOisn indicates that the coordination sphere of RuNOisn remains intact during film production. Exposure of CSx-RuNOisn to long wave UV-light (λirr = 355 nm) leads to NO release and formation of the paramagnetic photoproduct trans-[RuIII(NH3)4isn(H2O)]3+ in the CS film. Reaction of this aquaruthenium(iii) complex with aqueous nitrite regenerates RuNOisn in the film. Delivery of NO upon photolysis of CSx-RuNOisn was verified by trapping with oxymyoglobin. Moreover, NO release upon chemical reduction was carried out using l-cysteine as a reductant. Cysteine-mediated NO delivery from CSx-RuNOisn persisted for more than 7 h, during which physiologically relevant NO concentrations were liberated. These results suggest that CSx-RuNOisn is a promising candidate for use in biological applications.
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U2 - 10.1039/c4tb00996g
DO - 10.1039/c4tb00996g
M3 - Article
AN - SCOPUS:84907657421
SN - 2050-7518
VL - 2
SP - 7232
EP - 7242
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 41
ER -