Light microscopic autoradiographic localization of [3H]pirenzepine and [3H](-)quinuclidinyl benzilate binding in human stellate ganglia

Henry I. Yamamura; Mark Watson; James K. Wamsley; Peter C. Johnson; William R. Roeske

doi:10.1016/0024-3205(84)90344-8

Light microscopic autoradiographic localization of [³H]pirenzepine and [³H](-)quinuclidinyl benzilate binding in human stellate ganglia

Henry I. Yamamura, Mark Watson, James K. Wamsley, Peter C. Johnson, William R. Roeske

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We have utilized the LKB Ultrofilm method of autoradiography to anatomically localize putative M₁ and M₂ muscarinic receptor subtypes in human stellate ganglia. Ten micron sections were labeled in vitro with either 1 nM of the classical antagonist [³H](-)quinuclidinyl benzilate ([³H](-)QNB) or 20 nM of the non-classical antagonist [³H]pirenzepine ([³H]PZ), using 1 μM atropine sulfate to define non-specific binding for both ligands. Our results indicate that [³H](-)QNB and [³H]PZ binding sites are distributed within the principal ganglion cells and nerve bundles.

Original language	English (US)
Pages (from-to)	753-757
Number of pages	5
Journal	Life Sciences
Volume	35
Issue number	7
DOIs	https://doi.org/10.1016/0024-3205(84)90344-8
State	Published - Aug 13 1984

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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@article{9d420b35561c4f71aebab24acca41d21,

title = "Light microscopic autoradiographic localization of [3H]pirenzepine and [3H](-)quinuclidinyl benzilate binding in human stellate ganglia",

abstract = "We have utilized the LKB Ultrofilm method of autoradiography to anatomically localize putative M1 and M2 muscarinic receptor subtypes in human stellate ganglia. Ten micron sections were labeled in vitro with either 1 nM of the classical antagonist [3H](-)quinuclidinyl benzilate ([3H](-)QNB) or 20 nM of the non-classical antagonist [3H]pirenzepine ([3H]PZ), using 1 μM atropine sulfate to define non-specific binding for both ligands. Our results indicate that [3H](-)QNB and [3H]PZ binding sites are distributed within the principal ganglion cells and nerve bundles.",

author = "Yamamura, {Henry I.} and Mark Watson and Wamsley, {James K.} and Johnson, {Peter C.} and Roeske, {William R.}",

note = "Funding Information: Mark Watson is an awardee of a postdoctoral fellowship grant from the American Heart Association, Arizona Affiliate. Portions of this study were supported by USPHS grants ~H-30626, MH-27257, HL-29565 Program Project Grant HL-20984, a RSDA Type II (MH-O0095) from NIMH to Henry I. Yamamura, and a RCDA (HL-00776) from NHLBI to William R. Roeske. We thank Pat Gonzalez for secretarial assistance and Martha Ackerman for excellent technical help.",

year = "1984",

month = aug,

day = "13",

doi = "10.1016/0024-3205(84)90344-8",

language = "English (US)",

volume = "35",

pages = "753--757",

journal = "Life Sciences",

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TY - JOUR

T1 - Light microscopic autoradiographic localization of [3H]pirenzepine and [3H](-)quinuclidinyl benzilate binding in human stellate ganglia

AU - Yamamura, Henry I.

AU - Watson, Mark

AU - Wamsley, James K.

AU - Johnson, Peter C.

AU - Roeske, William R.

N1 - Funding Information: Mark Watson is an awardee of a postdoctoral fellowship grant from the American Heart Association, Arizona Affiliate. Portions of this study were supported by USPHS grants ~H-30626, MH-27257, HL-29565 Program Project Grant HL-20984, a RSDA Type II (MH-O0095) from NIMH to Henry I. Yamamura, and a RCDA (HL-00776) from NHLBI to William R. Roeske. We thank Pat Gonzalez for secretarial assistance and Martha Ackerman for excellent technical help.

PY - 1984/8/13

Y1 - 1984/8/13

N2 - We have utilized the LKB Ultrofilm method of autoradiography to anatomically localize putative M1 and M2 muscarinic receptor subtypes in human stellate ganglia. Ten micron sections were labeled in vitro with either 1 nM of the classical antagonist [3H](-)quinuclidinyl benzilate ([3H](-)QNB) or 20 nM of the non-classical antagonist [3H]pirenzepine ([3H]PZ), using 1 μM atropine sulfate to define non-specific binding for both ligands. Our results indicate that [3H](-)QNB and [3H]PZ binding sites are distributed within the principal ganglion cells and nerve bundles.

AB - We have utilized the LKB Ultrofilm method of autoradiography to anatomically localize putative M1 and M2 muscarinic receptor subtypes in human stellate ganglia. Ten micron sections were labeled in vitro with either 1 nM of the classical antagonist [3H](-)quinuclidinyl benzilate ([3H](-)QNB) or 20 nM of the non-classical antagonist [3H]pirenzepine ([3H]PZ), using 1 μM atropine sulfate to define non-specific binding for both ligands. Our results indicate that [3H](-)QNB and [3H]PZ binding sites are distributed within the principal ganglion cells and nerve bundles.

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AN - SCOPUS:0021210357

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VL - 35

SP - 753

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JO - Life Sciences

JF - Life Sciences

IS - 7

ER -

Light microscopic autoradiographic localization of [³H]pirenzepine and [³H](-)quinuclidinyl benzilate binding in human stellate ganglia

Abstract

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