Lethality of inducible, meristem-localized ectopic β-glucuronidase expression in plants

Fushi Wen; Ho Hyung Woo; Ann M. Hirsch; Martha C. Hawes

doi:10.1007/BF02773343

Lethality of inducible, meristem-localized ectopic β-glucuronidase expression in plants

Fushi Wen, Ho Hyung Woo, Ann M. Hirsch, Martha C. Hawes

Environmental Science, Research

Research output: Contribution to journal › Review article › peer-review

9 Scopus citations

Abstract

GUSA from Escherichia coli, encoded by the uidA gene, has been successfully used as a plant reporter system for more than a decade with no reported deleterious effects. However, when expressed in coordination with a UDP-glucuronosyltransferase isolated from the root cap meristem of Pisum sativum (PsUGT1) at the onset of mitosis, GUSA expression was lethal in pea, alfalfa, and Arabidopsis thaliana. These unexpected results indicate that, under some circumstances, using GUSA in plants is incompatible with life and suggest that the cell-specific lethal phenotype might be useful in selecting for genes specifically involved in regulating the G2-M phase of the cell cycle.

Original language	English (US)
Pages (from-to)	7-14
Number of pages	8
Journal	Plant Molecular Biology Reporter
Volume	22
Issue number	1
DOIs	https://doi.org/10.1007/BF02773343
State	Published - Mar 2004

Keywords

Cell cycle
Glucuronidase
Lethality
Meristem
Reporter gene
Root cap

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02773343

Cite this

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title = "Lethality of inducible, meristem-localized ectopic β-glucuronidase expression in plants",

abstract = "GUSA from Escherichia coli, encoded by the uidA gene, has been successfully used as a plant reporter system for more than a decade with no reported deleterious effects. However, when expressed in coordination with a UDP-glucuronosyltransferase isolated from the root cap meristem of Pisum sativum (PsUGT1) at the onset of mitosis, GUSA expression was lethal in pea, alfalfa, and Arabidopsis thaliana. These unexpected results indicate that, under some circumstances, using GUSA in plants is incompatible with life and suggest that the cell-specific lethal phenotype might be useful in selecting for genes specifically involved in regulating the G2-M phase of the cell cycle.",

keywords = "Cell cycle, Glucuronidase, Lethality, Meristem, Reporter gene, Root cap",

author = "Fushi Wen and Woo, {Ho Hyung} and Hirsch, {Ann M.} and Hawes, {Martha C.}",

note = "Funding Information: We acknowledge support from grants to M.C.H. and H.H.W. from the Department of Energy, the US Department of Agriculture, the National Science Foundation, and the College of Agriculture and Life Sciences at the University of Arizona.",

year = "2004",

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doi = "10.1007/BF02773343",

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volume = "22",

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T1 - Lethality of inducible, meristem-localized ectopic β-glucuronidase expression in plants

AU - Wen, Fushi

AU - Woo, Ho Hyung

AU - Hirsch, Ann M.

AU - Hawes, Martha C.

N1 - Funding Information: We acknowledge support from grants to M.C.H. and H.H.W. from the Department of Energy, the US Department of Agriculture, the National Science Foundation, and the College of Agriculture and Life Sciences at the University of Arizona.

PY - 2004/3

Y1 - 2004/3

N2 - GUSA from Escherichia coli, encoded by the uidA gene, has been successfully used as a plant reporter system for more than a decade with no reported deleterious effects. However, when expressed in coordination with a UDP-glucuronosyltransferase isolated from the root cap meristem of Pisum sativum (PsUGT1) at the onset of mitosis, GUSA expression was lethal in pea, alfalfa, and Arabidopsis thaliana. These unexpected results indicate that, under some circumstances, using GUSA in plants is incompatible with life and suggest that the cell-specific lethal phenotype might be useful in selecting for genes specifically involved in regulating the G2-M phase of the cell cycle.

AB - GUSA from Escherichia coli, encoded by the uidA gene, has been successfully used as a plant reporter system for more than a decade with no reported deleterious effects. However, when expressed in coordination with a UDP-glucuronosyltransferase isolated from the root cap meristem of Pisum sativum (PsUGT1) at the onset of mitosis, GUSA expression was lethal in pea, alfalfa, and Arabidopsis thaliana. These unexpected results indicate that, under some circumstances, using GUSA in plants is incompatible with life and suggest that the cell-specific lethal phenotype might be useful in selecting for genes specifically involved in regulating the G2-M phase of the cell cycle.

KW - Cell cycle

KW - Glucuronidase

KW - Lethality

KW - Meristem

KW - Reporter gene

KW - Root cap

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Lethality of inducible, meristem-localized ectopic β-glucuronidase expression in plants

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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