Lens cation transport and permeability changes following exposure to hydrogen peroxide

Nicholas A. Delamere; Christoper A. Paterson; Theodor R. Cotton

doi:10.1016/0014-4835(83)90148-3

Lens cation transport and permeability changes following exposure to hydrogen peroxide

Nicholas A. Delamere, Christoper A. Paterson, Theodor R. Cotton

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Exposure of the rabbit lens, in vitro, to 10^-3m-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10^-4 or 10^-5m-hydrogen peroxide. 10^-3m-hydrogen peroxide was observed to diminish ⁸⁶Rb uptake by 32% and to reduce the lens Na⁺-, K⁺-ATPase activity by 37%. The lens potential remained ouabain-sensitive after peroxide treatment. These findings suggest that only partial inhibition of the lens sodium pump takes place in the presence of 10^-3m-hydrogen peroxide. Lens depolarization, increased lens conductance and an increase rate of ⁸⁶Rb efflux were also observed upon exposure of the lens to 10^-3m-hydrogen peroxide. Such alterations of ⁸⁶Rb efflux and electrophysiological parameters indicate that hydrogen peroxide changes the passive permeability of lens membranes.

Original language	English (US)
Pages (from-to)	45-53
Number of pages	9
Journal	Experimental eye research
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0014-4835(83)90148-3
State	Published - Jul 1983
Externally published	Yes

Keywords

Na-, K-ATPase
active transport
electrolyte balance
hydrogen peroxide
membrane permeability
rabbit lens

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/0014-4835(83)90148-3

Cite this

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title = "Lens cation transport and permeability changes following exposure to hydrogen peroxide",

abstract = "Exposure of the rabbit lens, in vitro, to 10-3m-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10-4 or 10-5m-hydrogen peroxide. 10-3m-hydrogen peroxide was observed to diminish 86Rb uptake by 32% and to reduce the lens Na+-, K+-ATPase activity by 37%. The lens potential remained ouabain-sensitive after peroxide treatment. These findings suggest that only partial inhibition of the lens sodium pump takes place in the presence of 10-3m-hydrogen peroxide. Lens depolarization, increased lens conductance and an increase rate of 86Rb efflux were also observed upon exposure of the lens to 10-3m-hydrogen peroxide. Such alterations of 86Rb efflux and electrophysiological parameters indicate that hydrogen peroxide changes the passive permeability of lens membranes.",

keywords = "Na-, K-ATPase, active transport, electrolyte balance, hydrogen peroxide, membrane permeability, rabbit lens",

author = "Delamere, {Nicholas A.} and Paterson, {Christoper A.} and Cotton, {Theodor R.}",

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T1 - Lens cation transport and permeability changes following exposure to hydrogen peroxide

AU - Delamere, Nicholas A.

AU - Paterson, Christoper A.

AU - Cotton, Theodor R.

PY - 1983/7

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N2 - Exposure of the rabbit lens, in vitro, to 10-3m-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10-4 or 10-5m-hydrogen peroxide. 10-3m-hydrogen peroxide was observed to diminish 86Rb uptake by 32% and to reduce the lens Na+-, K+-ATPase activity by 37%. The lens potential remained ouabain-sensitive after peroxide treatment. These findings suggest that only partial inhibition of the lens sodium pump takes place in the presence of 10-3m-hydrogen peroxide. Lens depolarization, increased lens conductance and an increase rate of 86Rb efflux were also observed upon exposure of the lens to 10-3m-hydrogen peroxide. Such alterations of 86Rb efflux and electrophysiological parameters indicate that hydrogen peroxide changes the passive permeability of lens membranes.

AB - Exposure of the rabbit lens, in vitro, to 10-3m-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10-4 or 10-5m-hydrogen peroxide. 10-3m-hydrogen peroxide was observed to diminish 86Rb uptake by 32% and to reduce the lens Na+-, K+-ATPase activity by 37%. The lens potential remained ouabain-sensitive after peroxide treatment. These findings suggest that only partial inhibition of the lens sodium pump takes place in the presence of 10-3m-hydrogen peroxide. Lens depolarization, increased lens conductance and an increase rate of 86Rb efflux were also observed upon exposure of the lens to 10-3m-hydrogen peroxide. Such alterations of 86Rb efflux and electrophysiological parameters indicate that hydrogen peroxide changes the passive permeability of lens membranes.

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KW - active transport

KW - electrolyte balance

KW - hydrogen peroxide

KW - membrane permeability

KW - rabbit lens

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Lens cation transport and permeability changes following exposure to hydrogen peroxide

Abstract

Keywords

ASJC Scopus subject areas

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