Lens cation transport and permeability changes following exposure to hydrogen peroxide

Nicholas A. Delamere, Christoper A. Paterson, Theodor R. Cotton

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Exposure of the rabbit lens, in vitro, to 10-3m-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10-4 or 10-5m-hydrogen peroxide. 10-3m-hydrogen peroxide was observed to diminish 86Rb uptake by 32% and to reduce the lens Na+-, K+-ATPase activity by 37%. The lens potential remained ouabain-sensitive after peroxide treatment. These findings suggest that only partial inhibition of the lens sodium pump takes place in the presence of 10-3m-hydrogen peroxide. Lens depolarization, increased lens conductance and an increase rate of 86Rb efflux were also observed upon exposure of the lens to 10-3m-hydrogen peroxide. Such alterations of 86Rb efflux and electrophysiological parameters indicate that hydrogen peroxide changes the passive permeability of lens membranes.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalExperimental eye research
Issue number1
StatePublished - Jul 1983


  • Na-, K-ATPase
  • active transport
  • electrolyte balance
  • hydrogen peroxide
  • membrane permeability
  • rabbit lens

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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