Lecithin-based novel cationic nanocarriers (LeciPlex) I: Fabrication, characterization and evaluation

Abhijit A. Date; Deepika Srivastava; Mangal S. Nagarsenker; Rita Mulherkar; Lata Panicker; Vinod Aswal; Puthusserickal A. Hassan; Frank Steiniger; Jana Thamm; Alfred Fahr

doi:10.2217/nnm.11.38

Lecithin-based novel cationic nanocarriers (LeciPlex) I: Fabrication, characterization and evaluation

Abhijit A. Date
, Deepika Srivastava
, Mangal S. Nagarsenker
, Rita Mulherkar
, Lata Panicker
, Vinod Aswal
, Puthusserickal A. Hassan
, Frank Steiniger
, Jana Thamm
, Alfred Fahr

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Aims: In the present investigation, the feasibility of fabricating novel self-assembled cationic nanocarriers (LeciPlex) containing cetyltrimethylammonium bromide (CTAB) or didodecyldimethylammonium bromide (DDAB) and soybean lecithin using pharmaceutically acceptable biocompatible solvents such as 2-Pyrrolidone (Soluphor P ^®) and diethyleneglycol monoethyl ether (Transcutol ^®) was established. Materials & Methods: The interaction between DDAB/CTAB and soybean lecithin in the nanocarriers was confirmed by differential scanning calorimetry and in vitro antimicrobial studies. The positive charge on the nanocarriers was confirmed by zeta potential analysis. Results: Transmission electron microscopy analysis could not reveal sufficient information regarding the internal structure of the nanocarriers, whereas cryotransmission electron microscopy studies indicated that these novel nanocarriers have unilamellar structure. Small-angle neutron scattering studies confirmed interaction of cationic surfactant (DDAB) and lecithin in the nanocarriers and confirmed the presence of unilamellar nanostructures. Conclusion: Various hydrophobic drugs could be encapsulated in the CTAB/DDAB-based lecithin nanocarriers (CTAB-LeciPlex or DDAB-LeciPlex) irrespective of their difference in log p-values. In vitro antimicrobial studies on triclosan-loaded LeciPlex confirmed entrapment of triclosan in the nanocarriers. The ability of CTAB-LeciPlex and DDAB-LeciPlex to condense plasmid DNA was established using agarose gel electrophoresis. DDAB-LeciPlex could successfully transfect pDNA in HEK-293 cells indicating potential in gene delivery.

Original language	English (US)
Pages (from-to)	1309-1325
Number of pages	17
Journal	Nanomedicine
Volume	6
Issue number	8
DOIs	https://doi.org/10.2217/nnm.11.38
State	Published - Oct 2011
Externally published	Yes

Keywords

cationic nanocarrier
CTAB
DDAB
lecithin
SANS
Transcutol
transfection

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science
Development

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10.2217/nnm.11.38

Cite this

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title = "Lecithin-based novel cationic nanocarriers (LeciPlex) I: Fabrication, characterization and evaluation",

abstract = "Aims: In the present investigation, the feasibility of fabricating novel self-assembled cationic nanocarriers (LeciPlex) containing cetyltrimethylammonium bromide (CTAB) or didodecyldimethylammonium bromide (DDAB) and soybean lecithin using pharmaceutically acceptable biocompatible solvents such as 2-Pyrrolidone (Soluphor P {\textregistered}) and diethyleneglycol monoethyl ether (Transcutol {\textregistered}) was established. Materials \& Methods: The interaction between DDAB/CTAB and soybean lecithin in the nanocarriers was confirmed by differential scanning calorimetry and in vitro antimicrobial studies. The positive charge on the nanocarriers was confirmed by zeta potential analysis. Results: Transmission electron microscopy analysis could not reveal sufficient information regarding the internal structure of the nanocarriers, whereas cryotransmission electron microscopy studies indicated that these novel nanocarriers have unilamellar structure. Small-angle neutron scattering studies confirmed interaction of cationic surfactant (DDAB) and lecithin in the nanocarriers and confirmed the presence of unilamellar nanostructures. Conclusion: Various hydrophobic drugs could be encapsulated in the CTAB/DDAB-based lecithin nanocarriers (CTAB-LeciPlex or DDAB-LeciPlex) irrespective of their difference in log p-values. In vitro antimicrobial studies on triclosan-loaded LeciPlex confirmed entrapment of triclosan in the nanocarriers. The ability of CTAB-LeciPlex and DDAB-LeciPlex to condense plasmid DNA was established using agarose gel electrophoresis. DDAB-LeciPlex could successfully transfect pDNA in HEK-293 cells indicating potential in gene delivery.",

keywords = "cationic nanocarrier, CTAB, DDAB, lecithin, SANS, Transcutol, transfection",

author = "Date, \{Abhijit A.\} and Deepika Srivastava and Nagarsenker, \{Mangal S.\} and Rita Mulherkar and Lata Panicker and Vinod Aswal and Hassan, \{Puthusserickal A.\} and Frank Steiniger and Jana Thamm and Alfred Fahr",

year = "2011",

month = oct,

doi = "10.2217/nnm.11.38",

language = "English (US)",

volume = "6",

pages = "1309--1325",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Taylor and Francis Ltd.",

number = "8",

}

TY - JOUR

T1 - Lecithin-based novel cationic nanocarriers (LeciPlex) I

T2 - Fabrication, characterization and evaluation

AU - Date, Abhijit A.

AU - Srivastava, Deepika

AU - Nagarsenker, Mangal S.

AU - Mulherkar, Rita

AU - Panicker, Lata

AU - Aswal, Vinod

AU - Hassan, Puthusserickal A.

AU - Steiniger, Frank

AU - Thamm, Jana

AU - Fahr, Alfred

PY - 2011/10

Y1 - 2011/10

N2 - Aims: In the present investigation, the feasibility of fabricating novel self-assembled cationic nanocarriers (LeciPlex) containing cetyltrimethylammonium bromide (CTAB) or didodecyldimethylammonium bromide (DDAB) and soybean lecithin using pharmaceutically acceptable biocompatible solvents such as 2-Pyrrolidone (Soluphor P ®) and diethyleneglycol monoethyl ether (Transcutol ®) was established. Materials & Methods: The interaction between DDAB/CTAB and soybean lecithin in the nanocarriers was confirmed by differential scanning calorimetry and in vitro antimicrobial studies. The positive charge on the nanocarriers was confirmed by zeta potential analysis. Results: Transmission electron microscopy analysis could not reveal sufficient information regarding the internal structure of the nanocarriers, whereas cryotransmission electron microscopy studies indicated that these novel nanocarriers have unilamellar structure. Small-angle neutron scattering studies confirmed interaction of cationic surfactant (DDAB) and lecithin in the nanocarriers and confirmed the presence of unilamellar nanostructures. Conclusion: Various hydrophobic drugs could be encapsulated in the CTAB/DDAB-based lecithin nanocarriers (CTAB-LeciPlex or DDAB-LeciPlex) irrespective of their difference in log p-values. In vitro antimicrobial studies on triclosan-loaded LeciPlex confirmed entrapment of triclosan in the nanocarriers. The ability of CTAB-LeciPlex and DDAB-LeciPlex to condense plasmid DNA was established using agarose gel electrophoresis. DDAB-LeciPlex could successfully transfect pDNA in HEK-293 cells indicating potential in gene delivery.

AB - Aims: In the present investigation, the feasibility of fabricating novel self-assembled cationic nanocarriers (LeciPlex) containing cetyltrimethylammonium bromide (CTAB) or didodecyldimethylammonium bromide (DDAB) and soybean lecithin using pharmaceutically acceptable biocompatible solvents such as 2-Pyrrolidone (Soluphor P ®) and diethyleneglycol monoethyl ether (Transcutol ®) was established. Materials & Methods: The interaction between DDAB/CTAB and soybean lecithin in the nanocarriers was confirmed by differential scanning calorimetry and in vitro antimicrobial studies. The positive charge on the nanocarriers was confirmed by zeta potential analysis. Results: Transmission electron microscopy analysis could not reveal sufficient information regarding the internal structure of the nanocarriers, whereas cryotransmission electron microscopy studies indicated that these novel nanocarriers have unilamellar structure. Small-angle neutron scattering studies confirmed interaction of cationic surfactant (DDAB) and lecithin in the nanocarriers and confirmed the presence of unilamellar nanostructures. Conclusion: Various hydrophobic drugs could be encapsulated in the CTAB/DDAB-based lecithin nanocarriers (CTAB-LeciPlex or DDAB-LeciPlex) irrespective of their difference in log p-values. In vitro antimicrobial studies on triclosan-loaded LeciPlex confirmed entrapment of triclosan in the nanocarriers. The ability of CTAB-LeciPlex and DDAB-LeciPlex to condense plasmid DNA was established using agarose gel electrophoresis. DDAB-LeciPlex could successfully transfect pDNA in HEK-293 cells indicating potential in gene delivery.

KW - cationic nanocarrier

KW - CTAB

KW - DDAB

KW - lecithin

KW - SANS

KW - Transcutol

KW - transfection

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UR - https://www.scopus.com/pages/publications/79958272247#tab=citedBy

U2 - 10.2217/nnm.11.38

DO - 10.2217/nnm.11.38

M3 - Article

C2 - 22026377

AN - SCOPUS:79958272247

SN - 1743-5889

VL - 6

SP - 1309

EP - 1325

JO - Nanomedicine

JF - Nanomedicine

IS - 8

ER -

Lecithin-based novel cationic nanocarriers (LeciPlex) I: Fabrication, characterization and evaluation

Abstract

Keywords

ASJC Scopus subject areas

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