LC-ICP-OES method for antimony speciation analysis in liquid samples

Iván Moreno-Andrade, Enrique Regidor-Alfageme, Armando Durazo, Jim A. Field, Kelly Umlauf, Reyes Sierra-Alvarez

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


A method for the analysis of different species of antimony (Sb) that couples liquid chromatography with an inductively coupled plasma-optical emission spectrometry (LC-ICP-OES) system is presented. The method is simple and reliable to separate and quantify directly and simultaneously Sb(III) and Sb(V) in aqueous samples. The calibration curves showed high linearity at the three wavelengths tested. The limits of detection ranged from 24.9 to 32.3 μg/L for Sb(III) and from 36.2 to 46.0 μg/L for Sb(V), at the three wavelengths evaluated. The limit of detection for this method varied depending on the wavelength used. The lowest limit of quantification for Sb(V) (49.9 μg/L) and Sb(III) (80.7 μg/L) was obtained at a wavelength of 217.582 nm. The method sensitivity for Sb(V) was higher compared to Sb(III) at all the wavelengths considered. Samples containing different concentrations of Sb(III) and Sb(V) in three different matrices, i.e., water, basal culture medium, and anaerobic sludge plus basal medium, were analyzed. The coefficients of variation were low and ranged from 0.1 to 5.0 depending on the sample matrix. Recoveries of Sb(III) and Sb(V) were higher than 90% independently of the matrix analyzed and the wavelength used in the analysis.

Original languageEnglish (US)
Pages (from-to)457-463
Number of pages7
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Issue number4
StatePublished - Mar 20 2020


  • Antimony
  • Sb(III)
  • Sb(V)
  • hyphenated technique
  • speciation

ASJC Scopus subject areas

  • Environmental Engineering


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