@inproceedings{9168b471fb2d4f869f356f39ea6e93da,
title = "Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device",
abstract = "We have investigated the utilization of particle agglutination assays using forward light scattering measurements in a microfluidic device towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microspheres (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect live modified BVDV. This assay was first performed in a two well slide for proof of concept and then in a simple y-channel microfluidic device with optical fibers arranged in a close proximity setup. Particle immunoagglutination was detected through static light scattering measurements taken at 45° to incident light. In the microfluidic device, modified live BVDV was detected with a detection limit of 0.5 TCID50 mL-1.",
keywords = "BVD, BVDV, Immunoassay, Lab-on-a-chip, Microspheres, Proximity optical fibers",
author = "Heinze, {Brian C.} and Song, {Jae Young} and Han, {Jin Hee} and Yoon, {Jeong Yeol}",
year = "2008",
doi = "10.1117/12.761341",
language = "English (US)",
isbn = "9780819470614",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Microfluidics, BioMEMS, and Medical Microsystems VI",
note = "Microfluidics, BioMEMS, and Medical Microsystems VI ; Conference date: 21-01-2008 Through 22-01-2008",
}