Lateral diffusion of membrane-spanning and glycosylphosphatidylinositol-linked proteins: Toward establishing rules governing the lateral mobility of membrane proteins

F. Zhang, B. Crise, B. Su, Y. Hou, J. K. Rose, A. Bothwell, K. Jacobson

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

In the plasma membrane of animal cells, many membrane-spanning proteins exhibit lower lateral mobilities than glycosylphosphatidylinositol (GPI)-linked proteins. To determine if the GPI linkage was a major determinant of the high lateral mobility of these proteins, we measured the lateral diffusion of chimeric membrane proteins composed of normally transmembrane proteins that were converted to GPI-linked proteins, or GPI-linked proteins that were converted to membrane-spanning proteins. These studies indicate that GPI linkage contributes only marginally (approximately twofold) to the higher mobility of several GPI-linked proteins. The major determinant of the high mobility of these proteins resides instead in the extracellular domain. We propose that lack of interaction of the extracellular domain of this protein class with other cell surface components allows diffusion that is constrained only by the diffusion of the membrane anchor. In contrast, cell surface interactions of the ectodomain of membrane-spanning proteins exemplified by the vesicular stomatitis virus G glycoprotein reduces their lateral diffusion coefficients by nearly 10-fold with respect to many GPI-linked proteins.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalJournal of Cell Biology
Volume115
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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