TY - JOUR
T1 - Laser ablation methods for analysis of urinary calculi
T2 - Comparison study based on calibration pellets
AU - Štěpánková, K.
AU - Novotný, K.
AU - Vašinová Galiová, M.
AU - Kanický, V.
AU - Kaiser, J.
AU - Hahn, D. W.
N1 - Funding Information:
We acknowledge the Ministry of Education, Youth and Sports of the Czech Republic for bestowing the research projects ME08002, ME10061 and the Czech Science Foundation for the project GA203/09/1394. This work was also supported by the project “ CEITEC — Central European Institute of Technology ” ( CZ.1.05/1.1.00/02.0068 ) from the European Regional Development Fund . JK acknowledges the support of Brno University of Technology on the frame of grant FSI-S-11-22 (Application of advanced optical methods).
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Methods based on laser ablation, such as Laser-Induced Breakdown Spectroscopy (LIBS) and Laser-Ablation Inductively Coupled Plasma Mass/Optical Emission Spectrometry (LA-ICP-MS/OES) are particularly suitable for urinary calculi bulk and micro analysis. Investigation of spatial distribution of matrix and trace elements can help to explain their emergence and growth. However, quantification is still very problematic and these methods are often used only for qualitative elemental mapping. There are no commercially available standards, which would correspond to the urinary calculi matrix. Internal standardization is also difficult, mainly due to different crystalline phases in one kidney stone. The aim of this study is to demonstrate the calibration capabilities and examine the limitations of laser ablation based techniques. Calibration pellets were prepared from powdered human urinary calculi with phosphate, oxalate and urate matrix. For this comparative study, the most frequently used laser-ablation based analytical techniques were chosen, such as LIBS and LA-ICP-MS. Moreover, some alternative techniques such as simultaneous LIBS-LA-ICP-OES and laser ablation LA-LIBS were also utilized.
AB - Methods based on laser ablation, such as Laser-Induced Breakdown Spectroscopy (LIBS) and Laser-Ablation Inductively Coupled Plasma Mass/Optical Emission Spectrometry (LA-ICP-MS/OES) are particularly suitable for urinary calculi bulk and micro analysis. Investigation of spatial distribution of matrix and trace elements can help to explain their emergence and growth. However, quantification is still very problematic and these methods are often used only for qualitative elemental mapping. There are no commercially available standards, which would correspond to the urinary calculi matrix. Internal standardization is also difficult, mainly due to different crystalline phases in one kidney stone. The aim of this study is to demonstrate the calibration capabilities and examine the limitations of laser ablation based techniques. Calibration pellets were prepared from powdered human urinary calculi with phosphate, oxalate and urate matrix. For this comparative study, the most frequently used laser-ablation based analytical techniques were chosen, such as LIBS and LA-ICP-MS. Moreover, some alternative techniques such as simultaneous LIBS-LA-ICP-OES and laser ablation LA-LIBS were also utilized.
KW - LA-ICP-MS/OES
KW - LA-LIBS
KW - Laser ablation
KW - LIBS
KW - Urinary calculi
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U2 - 10.1016/j.sab.2012.12.009
DO - 10.1016/j.sab.2012.12.009
M3 - Article
AN - SCOPUS:84873713108
VL - 81
SP - 43
EP - 49
JO - Spectrochimica Acta, Part B: Atomic Spectroscopy
JF - Spectrochimica Acta, Part B: Atomic Spectroscopy
SN - 0584-8547
ER -