Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

Soroush Mehravar; Bhaskar Banerjee; Hemant Chatrath; Babak Amirsolaimani; Krunal Patel; Charmi Patel; Robert A. Norwood; Nasser Peyghambarian; Khanh Kieu

doi:10.1364/BOE.7.000148

Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

Soroush Mehravar, Bhaskar Banerjee, Hemant Chatrath, Babak Amirsolaimani, Krunal Patel, Charmi Patel, Robert A. Norwood, Nasser Peyghambarian, Khanh Kieu

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Barrett’s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett’s.

Original language	English (US)
Pages (from-to)	148-157
Number of pages	10
Journal	Biomedical Optics Express
Volume	7
Issue number	1
DOIs	https://doi.org/10.1364/BOE.7.000148
State	Published - Dec 16 2015

Keywords

Multiphoton processes
Nonlinear microscopy
Scanning microscopy
Ultrafast lasers

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

Access to Document

10.1364/BOE.7.000148

Cite this

@article{c5a6ef5893f74b428a9d3f49effd9c1c,

title = "Label-free multi-photon imaging of dysplasia in Barrett{\textquoteright}s esophagus",

abstract = "Barrett{\textquoteright}s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett{\textquoteright}s.",

keywords = "Multiphoton processes, Nonlinear microscopy, Scanning microscopy, Ultrafast lasers",

author = "Soroush Mehravar and Bhaskar Banerjee and Hemant Chatrath and Babak Amirsolaimani and Krunal Patel and Charmi Patel and Norwood, {Robert A.} and Nasser Peyghambarian and Khanh Kieu",

note = "Publisher Copyright: {\textcopyright}2015 Optical Society of America.",

year = "2015",

month = dec,

day = "16",

doi = "10.1364/BOE.7.000148",

language = "English (US)",

volume = "7",

pages = "148--157",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "The Optical Society",

number = "1",

}

TY - JOUR

T1 - Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

AU - Mehravar, Soroush

AU - Banerjee, Bhaskar

AU - Chatrath, Hemant

AU - Amirsolaimani, Babak

AU - Patel, Krunal

AU - Patel, Charmi

AU - Norwood, Robert A.

AU - Peyghambarian, Nasser

AU - Kieu, Khanh

PY - 2015/12/16

Y1 - 2015/12/16

N2 - Barrett’s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett’s.

AB - Barrett’s esophagus (BE) is a metaplastic disorder where dysplastic and early cancerous changes are invisible to the naked eye and where the practice of blind biopsy is hampered by large sampling errors. Multi-photon microscopy (MPM) has emerged as an alternative solution for fast and label-free diagnostic capability for identifying the histological features with sub-micron accuracy. We developed a compact, inexpensive MPM system by using a handheld mode-locked fiber laser operating at 1560nm to study mucosal biopsies of BE. The combination of backscattered THG, back-reflected forward THG and SHG signals generate images of cell nuclei and collagen, leading to label-free diagnosis in Barrett’s.

KW - Multiphoton processes

KW - Nonlinear microscopy

KW - Scanning microscopy

KW - Ultrafast lasers

UR - http://www.scopus.com/inward/record.url?scp=84961807041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961807041&partnerID=8YFLogxK

U2 - 10.1364/BOE.7.000148

DO - 10.1364/BOE.7.000148

M3 - Article

AN - SCOPUS:84961807041

SN - 2156-7085

VL - 7

SP - 148

EP - 157

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 1

ER -

Label-free multi-photon imaging of dysplasia in Barrett’s esophagus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this