L-ascorbic acid regulates growth and metabolism of renal cells: Improvements in cell culture

Grazyna Nowak, Rick G. Schnellmann

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

The addition of L-ascorbic acid 2-phosphate (AscP) to primary cultures of rabbit renal proximal tubular cells (RPTC) grown under improved culture conditions resulted in an extended growth phase and increased cellular density (1.3-fold increase in monolayer DNA and protein contents). AscP reduced glycolysis, increased net lactate consumption by 38%, and stimulated net glucose production by 47%. Basal O2 consumption increased by 39% in RPTC grown in the presence of AscP and was equivalent to that in freshly isolated proximal tubules. AscP increased ouabain-sensitive O2 consumption (81%) and Na+-K+-ATPase activity (2.5-fold), which suggested increased active Na+ transport. Addition of AscP increased Na+-dependent glucose uptake by 43% and brush-border enzyme marker activities by 46%. It is concluded that supplementation of media with AscP further improves RPTC culture conditions by promotion of cellular growth and stimulation of in vivo-like respiration, lactate utilization, and net glucose synthesis. These changes are accompanied by an increase in brush-border enzyme activities and stimulation of active Na+ transport and Na+-dependent glucose transport, which demonstrate an improved expression of brush-border membrane functions in RPTC.

Original languageEnglish (US)
Pages (from-to)C2072-C2080
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number6 40-6
DOIs
StatePublished - Dec 1996
Externally publishedYes

Keywords

  • active sodium transport
  • basal and ouabain-sensitive oxygen consumption
  • gluconeogenesis
  • glycolysis
  • lactate consumption
  • renal proximal tubular cells
  • sodium-coupled glucose transport
  • sodium-potassium-adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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