TY - JOUR
T1 - KLU suppresses megasporocyte cell fate through SWR1-mediated activation of WRKY28 expression in Arabidopsis
AU - Zhao, Lihua
AU - Cai, Hanyang
AU - Su, Zhenxia
AU - Wang, Lulu
AU - Huang, Xinyu
AU - Zhang, Man
AU - Chen, Piaojuan
AU - Dai, Xiaozhuan
AU - Zhao, Heming
AU - Palanivelu, Ravishankar
AU - Chen, Xuemei
AU - Qin, Yuan
N1 - Funding Information:
We thank Dr. W. Yang for sharing the pAKV:H2B-YFP marker, R. Yadegari for providing the pDMC1:GFP marker, R. Deal and R. Meagher for sharing the H2A.Z and ARP6 antibodies, Dr. B. Sun for the pKNU:KNU-Venus line, and Z. Yang, L. Xia, and I. Lavagi for critical reading. This work was supported by National Natural Science Foundation of China (NSFC) Grants 31470284 (to Y.Q.), 31522009 (to Y.Q.), U1605212 (to Y.Q.), 31761130074 (to Y.Q.), 31600249 (to L.Z.), 31571264 (to H.Z.), and 31700278 (to Z.S.), Newton Advanced Fellowship NA160391 (to Y.Q.), and Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University Grant 1122yb035 (to L.Z.).
Funding Information:
ACKNOWLEDGMENTS. We thank Dr. W. Yang for sharing the pAKV:H2B-YFP marker, R. Yadegari for providing the pDMC1:GFP marker, R. Deal and R. Meagher for sharing the H2A.Z and ARP6 antibodies, Dr. B. Sun for the pKNU:KNU-Venus line, and Z. Yang, L. Xia, and I. Lavagi for critical reading. This work was supported by National Natural Science Foundation of China (NSFC) Grants 31470284 (to Y.Q.), 31522009 (to Y.Q.), U1605212 (to Y.Q.), 31761130074 (to Y.Q.), 31600249 (to L.Z.), 31571264 (to H.Z.), and 31700278 (to Z.S.), Newton Advanced Fellowship NA160391 (to Y.Q.), and Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University Grant 1122yb035 (to L.Z.).
PY - 2018/1/16
Y1 - 2018/1/16
N2 - Germ-line specification is essential for sexual reproduction. In the ovules of most flowering plants, only a single hypodermal cell enlarges and differentiates into a megaspore mother cell (MMC), the founder cell of the female germ-line lineage. The molecular mechanisms restricting MMC specification to a single cell remain elusive. We show that the Arabidopsis transcription factor WRKY28 is exclusively expressed in hypodermal somatic cells surrounding the MMC and is required to repress these cells from acquiring MMC-like cell identity. In this process, the SWR1 chromatin remodeling complex mediates the incorporation of the histone variant H2A.Z at the WRKY28 locus. Moreover, the cytochrome P450 gene KLU, expressed in inner integument primordia, non–cell-autonomously promotes WRKY28 expression through H2A.Z deposition at WRKY28. Taken together, our findings show how somatic cells in ovule primordia cooperatively use chromatin remodeling to restrict germ-line cell specification to a single cell.
AB - Germ-line specification is essential for sexual reproduction. In the ovules of most flowering plants, only a single hypodermal cell enlarges and differentiates into a megaspore mother cell (MMC), the founder cell of the female germ-line lineage. The molecular mechanisms restricting MMC specification to a single cell remain elusive. We show that the Arabidopsis transcription factor WRKY28 is exclusively expressed in hypodermal somatic cells surrounding the MMC and is required to repress these cells from acquiring MMC-like cell identity. In this process, the SWR1 chromatin remodeling complex mediates the incorporation of the histone variant H2A.Z at the WRKY28 locus. Moreover, the cytochrome P450 gene KLU, expressed in inner integument primordia, non–cell-autonomously promotes WRKY28 expression through H2A.Z deposition at WRKY28. Taken together, our findings show how somatic cells in ovule primordia cooperatively use chromatin remodeling to restrict germ-line cell specification to a single cell.
KW - Cytochrome P450 KLU
KW - Megaspore mother cell
KW - SWR1 |
KW - WRKY28
KW - |
UR - http://www.scopus.com/inward/record.url?scp=85040629468&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040629468&partnerID=8YFLogxK
U2 - 10.1073/pnas.1716054115
DO - 10.1073/pnas.1716054115
M3 - Article
C2 - 29288215
AN - SCOPUS:85040629468
SN - 0027-8424
VL - 115
SP - E526-E535
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3
ER -