Kinetics of interleukin-1 and tumor necrosis factor secretion by rabbit macrophages recovered from the peritoneal cavity after surgery

Macrophages play a crucial role in wound healing after surgical injury, both as scavenger cells responsible for wound debridement and as cells that secrete soluble factors such as interleukin-1 (IL-1) and tumor necrosis factor (TNF). IL-1 and TNF alter many of the biological activities of cells that appear in postsurgical wounds. In this study, we determined the kinetics of IL-1 and TNF production by rabbit macrophages harvested from postsurgical peritoneal exudate (postsurgical macrophages) at several time points after peritoneal surgery. To further characterize the level of functional activities of postsurgical macrophages, the IL-1 and TNF levels were determined with or without stimulating the cells with lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). After surgery, the number of macrophages harvested by peritoneal lavage increased, reached peak levels on postsurgical day 3, and then decreased. IL-1 levels secreted by macrophages cultured without stimuli were elevated on postsurgical day 14 compared to the values on day 3 and 7. TNF concentrations peaked on days 1 and 14. In the conditioned culture media from LPS-PMA-stimulated macrophages, the levels of both IL-1 and TNF peaked on postsurgical days 3 and 14. These data suggest that the susceptibility of postsurgical macrophages to stimuli changes during the wound healing process with maximum sensitivity to the stimuli present during the early phase of peritoneal repair (day 3)