TY - JOUR
T1 - Just the two of us? A family of pseudomonas megaplasmids offers a rare glimpse into the evolution of largemobile elements
AU - Smith, Brian A.
AU - Leligdon, Courtney
AU - Baltrus, David A.
N1 - Publisher Copyright:
© 2019 The Author(s). Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Pseudomonads are ubiquitous group of environmental proteobacteria, well known for their roles in biogeochemical cycling, in the breakdown of xenobioticmaterials, as plant growth promoters, and as pathogens of a variety of host organisms.We have previously identified a largemegaplasmid present within one isolate of the plant pathogen Pseudomonas syringae, and here we report that a second member of this megaplasmid family is found within an environmental Pseudomonad isolate most closely related to Pseudomonas putida.Many of the shared genes are involved in critical cellular processes like replication, transcription, translation, and DNA repair. We argue that presence of these shared pathways sheds new light on discussions about the types of genes that undergo horizontal gene transfer (i.e., the complexity hypothesis) as well as the evolution of pangenomes. Furthermore, although bothmegaplasmids display a high level of synteny, genes that are shared differ by over 50%on average at the amino acid level. This combination of conservation in gene order despite divergence in gene sequence suggests that this Pseudomonad megaplasmid family is relatively old, that geneorder is under strongselectionwithinthis family, and that there are likelymanymoremembersof this megaplasmid family waiting to be found in nature.
AB - Pseudomonads are ubiquitous group of environmental proteobacteria, well known for their roles in biogeochemical cycling, in the breakdown of xenobioticmaterials, as plant growth promoters, and as pathogens of a variety of host organisms.We have previously identified a largemegaplasmid present within one isolate of the plant pathogen Pseudomonas syringae, and here we report that a second member of this megaplasmid family is found within an environmental Pseudomonad isolate most closely related to Pseudomonas putida.Many of the shared genes are involved in critical cellular processes like replication, transcription, translation, and DNA repair. We argue that presence of these shared pathways sheds new light on discussions about the types of genes that undergo horizontal gene transfer (i.e., the complexity hypothesis) as well as the evolution of pangenomes. Furthermore, although bothmegaplasmids display a high level of synteny, genes that are shared differ by over 50%on average at the amino acid level. This combination of conservation in gene order despite divergence in gene sequence suggests that this Pseudomonad megaplasmid family is relatively old, that geneorder is under strongselectionwithinthis family, and that there are likelymanymoremembersof this megaplasmid family waiting to be found in nature.
KW - Comparative genomics
KW - Complexity hypothesis
KW - Horizontal gene transfer
KW - Megaplasmid
KW - Pangenome
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U2 - 10.1093/gbe/evz066
DO - 10.1093/gbe/evz066
M3 - Article
C2 - 30918968
AN - SCOPUS:85065348630
SN - 1759-6653
VL - 11
SP - 1223
EP - 1234
JO - Genome biology and evolution
JF - Genome biology and evolution
IS - 4
ER -