JAGGER localization and function are dependent on GPI anchor addition

Raquel Figueiredo, Mónica Costa, Diana Moreira, Miguel Moreira, Jennifer Noble, Luís Gustavo Pereira, Paula Melo, Ravishankar Palanivelu, Sílvia Coimbra, Ana Marta Pereira

Research output: Contribution to journalArticlepeer-review

Abstract

Key message: GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct GPI anchor addition in JAGGER, negatively affects its localization and function. Abstract: In flowering plants, successful double fertilization requires the correct delivery of two sperm cells to the female gametophyte inside the ovule. The delivery of a single pair of sperm cells is achieved by the entrance of a single pollen tube into one female gametophyte. To prevent polyspermy, Arabidopsis ovules avoid the attraction of multiple pollen tubes to one ovule–polytubey block. In Arabidopsis jagger mutants, a significant number of ovules attract more than one pollen tube to an ovule due to an impairment in synergid degeneration. JAGGER encodes a putative arabinogalactan protein which is predicted to be anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Here, we show that JAGGER fused to citrine yellow fluorescent protein (JAGGER-cYFP) is functional and localizes mostly to the periphery of ovule integuments and transmitting tract cells. We further investigated the importance of GPI-anchor addition domains for JAGGER localization and function. Different JAGGER proteins with deletions in predicted ω-site regions and GPI attachment signal domain, expected to compromise the addition of the GPI anchor, led to disruption of JAGGER localization in the cell periphery. All JAGGER proteins with disrupted localization were also not able to rescue the polytubey phenotype, pointing to the importance of GPI-anchor addition to in vivo function of the JAGGER protein.

Original languageEnglish (US)
JournalPlant Reproduction
DOIs
StateAccepted/In press - 2024

Keywords

  • Arabidopsis
  • Arabinogalactan proteins (AGPs)
  • GPI-anchored proteins (GPI-APs)
  • Pollen–pistil interactions
  • Polytubey

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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