IV. Yeast sequencing reports. GUF1, a gene encoding a novel evolutionarily conserved gtpase in budding yeast

Gretchen L. Kiser; Ted A. Weinert

doi:10.1002/yea.320111312

IV. Yeast sequencing reports. GUF1, a gene encoding a novel evolutionarily conserved gtpase in budding yeast

Gretchen L. Kiser, Ted A. Weinert

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20 Scopus citations

Abstract

While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor‐type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236·1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1δ deletion and a putative constitutive‐activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype. The GenBank Accession Number for the GUF1 sequence is U22360.

Original language	English (US)
Pages (from-to)	1311-1316
Number of pages	6
Journal	Yeast
Volume	11
Issue number	13
DOIs	https://doi.org/10.1002/yea.320111312
State	Published - Oct 1995

Keywords

GTPase
GUF1
Saccharomyces cerevisiae
chromosome IV

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Genetics

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10.1002/yea.320111312

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abstract = "While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor‐type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236·1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1δ deletion and a putative constitutive‐activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype. The GenBank Accession Number for the GUF1 sequence is U22360.",

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AB - While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor‐type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236·1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1δ deletion and a putative constitutive‐activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype. The GenBank Accession Number for the GUF1 sequence is U22360.

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IV. Yeast sequencing reports. GUF1, a gene encoding a novel evolutionarily conserved gtpase in budding yeast

Abstract

Keywords

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