ITE Suppresses Angiogenic Responses in Human Artery and Vein Endothelial Cells: Differential Roles of AhR

Yan Li, Kai Wang, Qing yun Zou, Yi zhou Jiang, Chi Zhou, Jing Zheng

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor is involved in regulation of many essential biological processes including vascular development and angiogenesis. 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) is an AhR ligand, which regulates immune responses and cancer cell growth. However, the roles of the ITE/AhR pathway in mediating placental angiogenesis remains elusive. Here, we determined if ITE affected placental angiogenic responses via AhR in human umbilical vein (HUVECs) and artery endothelial (HUAECs) cells in vitro. We observed that ITE dose- and time-dependently inhibited proliferation and viability of HUAECs and HUVECs, whereas it inhibited migration of HUAECs, but not HUVECs. While AhR siRNA significantly suppressed AhR protein expression in HUVECs and HUAECs, it attenuated the ITE-inhibited angiogenic responses of HUAECs, but not HUVECs. Collectively, ITE suppressed angiogenic responses of HUAECs and HUVECs, dependent and independent of AhR, respectively. These data suggest that ITE may regulate placental angiogenesis.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalReproductive Toxicology
Volume74
DOIs
StatePublished - Dec 2017
Externally publishedYes

Keywords

  • Angiogenesis
  • Aryl hydrocarbon receptor
  • Endothelial cells
  • ITE

ASJC Scopus subject areas

  • Toxicology

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