@inbook{357d18a57f094a889c01168f6ee058e6,
title = "Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture",
abstract = "The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier, provides nutrients, recycles visual pigment, and removes spent discs from the photoreceptors, among many other functions. Because of these critical roles in visual homeostasis, the RPE is a principal location of disease-associated changes in age-related macular degeneration (AMD), emphasizing its importance for study in both visual health and disease. Unfortunately, there are no early indicators of AMD or disease progression, a void that could be filled by the development of early AMD biomarkers. Exosomes are lipid bilayer membrane vesicles of nanoscale sizes that are released in a controlled fashion by cells and carry out a number of extra- and intercellular activities. In the RPE they are released from both the apical and basal sides, and each source has a unique signature/content. Exosomes released from the basolateral side of RPE cells enter the systemic circulation via the choroid and thus represent a potential source of retinal disease biomarkers in blood. Here we discuss the potential of targeted immunocapture of eye-derived exosomes and other small extracellular vesicles from blood for eye disease biomarker discovery.",
keywords = "AMD, Age-related macular degeneration, Biomarker, Exosome, Extracellular vesicle, Immunocapture, Polarized, RPE, Retinal pigment epithelium",
author = "Mikael Klingeborn and Skiba, {Nikolai P.} and Stamer, {W. Daniel} and {Bowes Rickman}, Catherine",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Nature Switzerland AG.",
year = "2019",
doi = "10.1007/978-3-030-27378-1_4",
language = "English (US)",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer",
pages = "21--25",
booktitle = "Advances in Experimental Medicine and Biology",
}