Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

N. B. Egen; M. Bliss; M. Mayersohn; S. M. Owens; L. Arnold; M. Bier

doi:10.1016/0003-2697(88)90472-1

Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

N. B. Egen, M. Bliss, M. Mayersohn, S. M. Owens, L. Arnold, M. Bier

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Abstract

A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.

Original language	English (US)
Pages (from-to)	488-494
Number of pages	7
Journal	Analytical Biochemistry
Volume	172
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(88)90472-1
State	Published - Aug 1 1988

Keywords

Rotofor
ascites fluid
isoelectric focusing
monoclonal antibody
phencyclidine
purification

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(88)90472-1

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title = "Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor",

abstract = "A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.",

keywords = "Rotofor, ascites fluid, isoelectric focusing, monoclonal antibody, phencyclidine, purification",

author = "Egen, {N. B.} and M. Bliss and M. Mayersohn and Owens, {S. M.} and L. Arnold and M. Bier",

note = "Funding Information: This work was supported in part by NASA (NSG-7333 and NAGW-693) and the National Institute on Drug Abuse (DA 02680).",

year = "1988",

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doi = "10.1016/0003-2697(88)90472-1",

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T1 - Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

AU - Egen, N. B.

AU - Bliss, M.

AU - Mayersohn, M.

AU - Owens, S. M.

AU - Arnold, L.

AU - Bier, M.

N1 - Funding Information: This work was supported in part by NASA (NSG-7333 and NAGW-693) and the National Institute on Drug Abuse (DA 02680).

PY - 1988/8/1

Y1 - 1988/8/1

N2 - A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.

AB - A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.

KW - Rotofor

KW - ascites fluid

KW - isoelectric focusing

KW - monoclonal antibody

KW - phencyclidine

KW - purification

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ER -

Isolation of monoclonal antibodies to phencyclidine from ascites fluid by preparative isoelectric focusing in the Rotofor

Abstract

Keywords

ASJC Scopus subject areas

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