Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region in Peru

Ynes R. Ortega, Concepcion R. Roxas, Robert H. Gilman, Norma J. Miller, Lilia Cabrera, Carmen Taquiri, Charles R. Sterling

    Research output: Contribution to journalArticlepeer-review

    170 Scopus citations

    Abstract

    Cryptosporidium parvum and Cyclospora cayetanensis are protozoan pathogens that cause prolonged diarrhea in both immunocompetent and immunocompromised hosts. Cryptosporidium parvum can be transmitted via the fecal-oral route, while the exact mechanisms of transmission of Cyclospora cayetanensis have not been fully determined. Humans appear to be the sole host for the latter and a distinct seasonality has been observed in endemic areas around the world. Samples of vegetables were collected at several small markets in a periurban slum in Peru during the seasons of high and low incidence. The vegetables were washed, the supernatants were collected and centrifuged, and the pellets were resuspended in a solution of 2.5% potassium dichromate. Pellets were examined using direct microscopic observation, acid- fast staining, and immunofluorescent assays for C. parvum and Cyclospora cayetanensis oocysts. Samples were collected during three time periods: the season of low incidence, the beginning of the season of high incidence, and end of the season of high incidence. Of the total vegetables examined, 14.5% contained C. parvum oocysts and 1.8% had Cyclospora oocysts. Thus, market vegetables may provide a route by which Cryptosporidium and Cyclospora can be transmitted. Our study also suggests that washing vegetables does not completely remove Cryptosporidium and Cyclospora oocysts.

    Original languageEnglish (US)
    Pages (from-to)683-686
    Number of pages4
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume57
    Issue number6
    DOIs
    StatePublished - 1997

    ASJC Scopus subject areas

    • Parasitology
    • Virology
    • Infectious Diseases

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