@inbook{7dd3307a5f054bf18127caddcc5106bb,
title = "Isolation of a sequence-specific RNA binding protein, polypyrimidine tract binding protein, using RNA affinity chromatography",
abstract = "Many important cellular processes are mediated by sequence-specific RNA binding proteins, and it is often necessary to purify these proteins. When the RNA binding site is known, it is convenient to use this RNA as a matrix for affinity purification. The intronic splicing silencer (ISS) element present upstream of the N1 exon of the c-src pre-mRNA is a high-affinity binding site for the polypyrimidine tract binding protein (PTB). Using a 5′- biotinylated ISS RNA and PTB as an example, I describe a one-step method for affinity chromatography of RNA binding proteins from nuclear extracts.",
keywords = "Affinity chromatography, Intronic splicing silencer, Polypyrimidine tract binding protein, RNA binding proteins, c-src N1 exon",
author = "Shalini Sharma",
year = "2008",
doi = "10.1007/978-1-60327-475-3_1",
language = "English (US)",
isbn = "9781588294197",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "1--8",
booktitle = "RNA-Protein Interaction Protocols",
}