Isolation and screening of basidiomycetes with high peroxidative activity

Ed De Jong; Floris P. De Vries; Jim A. Field; Rick P. van der Zwan; Jan A.M. de Bont

doi:10.1016/S0953-7562(09)80121-4

Isolation and screening of basidiomycetes with high peroxidative activity

Ed De Jong, Floris P. De Vries, Jim A. Field, Rick P. van der Zwan, Jan A.M. de Bont

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Sixty-seven Poly R-478 decolorizing basidiomycetes were isolated with a selective medium (containing hemp (Cannabis sativa) stem wood, guaiacol and benomyl). Several of the new isolates seemed to be promising manganese peroxidase-containing white-rot fungi. Enzyme assays indicated that either glyoxal or veratryl alcohol oxidase were present in the culture fluids of peroxidative strains. In contrast, lignin peroxidase was only detected in Phanerochaete chrysosporium, despite attempts to induce this enzyme in other strains with oxygen and oxygen/veratryl alcohol additions. A highly significant correlation was found between two ligninolytic indicators: ethene formation from α-keto-γ-methylthiolbutyric acid and the decolorization of a polymeric dye, Poly R. Three of the new isolates had significantly higher Poly R decolorizing activities compared to P. chrysosporium. The Poly R decolorization rate is a good assay when trying to optimize culture conditions for peroxidase/H₂O₂ production.

Original language	English (US)
Pages (from-to)	1098-1104
Number of pages	7
Journal	Mycological Research
Volume	96
Issue number	12
DOIs	https://doi.org/10.1016/S0953-7562(09)80121-4
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Ecology, Evolution, Behavior and Systematics
Genetics
Plant Science

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title = "Isolation and screening of basidiomycetes with high peroxidative activity",

abstract = "Sixty-seven Poly R-478 decolorizing basidiomycetes were isolated with a selective medium (containing hemp (Cannabis sativa) stem wood, guaiacol and benomyl). Several of the new isolates seemed to be promising manganese peroxidase-containing white-rot fungi. Enzyme assays indicated that either glyoxal or veratryl alcohol oxidase were present in the culture fluids of peroxidative strains. In contrast, lignin peroxidase was only detected in Phanerochaete chrysosporium, despite attempts to induce this enzyme in other strains with oxygen and oxygen/veratryl alcohol additions. A highly significant correlation was found between two ligninolytic indicators: ethene formation from α-keto-γ-methylthiolbutyric acid and the decolorization of a polymeric dye, Poly R. Three of the new isolates had significantly higher Poly R decolorizing activities compared to P. chrysosporium. The Poly R decolorization rate is a good assay when trying to optimize culture conditions for peroxidase/H2O2 production.",

author = "{De Jong}, Ed and {De Vries}, {Floris P.} and Field, {Jim A.} and {van der Zwan}, {Rick P.} and {de Bont}, {Jan A.M.}",

year = "1992",

doi = "10.1016/S0953-7562(09)80121-4",

language = "English (US)",

volume = "96",

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journal = "Mycological Research",

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T1 - Isolation and screening of basidiomycetes with high peroxidative activity

AU - De Jong, Ed

AU - De Vries, Floris P.

AU - Field, Jim A.

AU - van der Zwan, Rick P.

AU - de Bont, Jan A.M.

PY - 1992

Y1 - 1992

N2 - Sixty-seven Poly R-478 decolorizing basidiomycetes were isolated with a selective medium (containing hemp (Cannabis sativa) stem wood, guaiacol and benomyl). Several of the new isolates seemed to be promising manganese peroxidase-containing white-rot fungi. Enzyme assays indicated that either glyoxal or veratryl alcohol oxidase were present in the culture fluids of peroxidative strains. In contrast, lignin peroxidase was only detected in Phanerochaete chrysosporium, despite attempts to induce this enzyme in other strains with oxygen and oxygen/veratryl alcohol additions. A highly significant correlation was found between two ligninolytic indicators: ethene formation from α-keto-γ-methylthiolbutyric acid and the decolorization of a polymeric dye, Poly R. Three of the new isolates had significantly higher Poly R decolorizing activities compared to P. chrysosporium. The Poly R decolorization rate is a good assay when trying to optimize culture conditions for peroxidase/H2O2 production.

AB - Sixty-seven Poly R-478 decolorizing basidiomycetes were isolated with a selective medium (containing hemp (Cannabis sativa) stem wood, guaiacol and benomyl). Several of the new isolates seemed to be promising manganese peroxidase-containing white-rot fungi. Enzyme assays indicated that either glyoxal or veratryl alcohol oxidase were present in the culture fluids of peroxidative strains. In contrast, lignin peroxidase was only detected in Phanerochaete chrysosporium, despite attempts to induce this enzyme in other strains with oxygen and oxygen/veratryl alcohol additions. A highly significant correlation was found between two ligninolytic indicators: ethene formation from α-keto-γ-methylthiolbutyric acid and the decolorization of a polymeric dye, Poly R. Three of the new isolates had significantly higher Poly R decolorizing activities compared to P. chrysosporium. The Poly R decolorization rate is a good assay when trying to optimize culture conditions for peroxidase/H2O2 production.

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DO - 10.1016/S0953-7562(09)80121-4

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VL - 96

SP - 1098

EP - 1104

JO - Mycological Research

JF - Mycological Research

IS - 12

ER -

Isolation and screening of basidiomycetes with high peroxidative activity

Abstract

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