Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources

I. P. Gladysheva; N. G. Balabushevich; N. A. Moroz; N. I. Larionova

Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources

I. P. Gladysheva, N. G. Balabushevich, N. A. Moroz, N. I. Larionova

Research output: Contribution to journal › Article › peer-review

Abstract

A chromatographic procedure for isolation of different isoforms of Bowman-Birk soybean trypsin inhibitors was developed. The number of isoforms was shown to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and processing products was analyzed. BBI reaches its highest concentration in freshly milled seeds. Storage conditions optimum for preservation of maximum inhibitory activity in soybean raw material were developed. The use of indirect enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.

Original language	English (US)
Pages (from-to)	198-203
Number of pages	6
Journal	Biochemistry (Moscow)
Volume	65
Issue number	2
State	Published - Feb 2000
Externally published	Yes

Keywords

Bowman-birk inhibitor
Proteinases
Soybean

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources",

abstract = "A chromatographic procedure for isolation of different isoforms of Bowman-Birk soybean trypsin inhibitors was developed. The number of isoforms was shown to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and processing products was analyzed. BBI reaches its highest concentration in freshly milled seeds. Storage conditions optimum for preservation of maximum inhibitory activity in soybean raw material were developed. The use of indirect enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.",

keywords = "Bowman-birk inhibitor, Proteinases, Soybean",

author = "Gladysheva, {I. P.} and Balabushevich, {N. G.} and Moroz, {N. A.} and Larionova, {N. I.}",

year = "2000",

month = feb,

language = "English (US)",

volume = "65",

pages = "198--203",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "Pleiades Publishing",

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TY - JOUR

T1 - Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources

AU - Gladysheva, I. P.

AU - Balabushevich, N. G.

AU - Moroz, N. A.

AU - Larionova, N. I.

PY - 2000/2

Y1 - 2000/2

N2 - A chromatographic procedure for isolation of different isoforms of Bowman-Birk soybean trypsin inhibitors was developed. The number of isoforms was shown to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and processing products was analyzed. BBI reaches its highest concentration in freshly milled seeds. Storage conditions optimum for preservation of maximum inhibitory activity in soybean raw material were developed. The use of indirect enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.

AB - A chromatographic procedure for isolation of different isoforms of Bowman-Birk soybean trypsin inhibitors was developed. The number of isoforms was shown to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and processing products was analyzed. BBI reaches its highest concentration in freshly milled seeds. Storage conditions optimum for preservation of maximum inhibitory activity in soybean raw material were developed. The use of indirect enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.

KW - Bowman-birk inhibitor

KW - Proteinases

KW - Soybean

UR - http://www.scopus.com/inward/record.url?scp=0034132819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034132819&partnerID=8YFLogxK

M3 - Article

C2 - 10713547

AN - SCOPUS:0034132819

SN - 0006-2979

VL - 65

SP - 198

EP - 203

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 2

ER -

Isolation and Characterization of Soybean Bowman-Birk Inhibitor from Different Sources

Abstract

Keywords

ASJC Scopus subject areas

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