Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta

Michael L. Siegel; Randy Horwitz; Timothy D. Morris; Raina M. Di Venere; Edward J. Siden

doi:10.1002/eji.1830151113

Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta

Michael L. Siegel, Randy Horwitz, Timothy D. Morris, Raina M. Di Venere, Edward J. Siden

Integrative Medicine, Andrew Weil Center for

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines express characteristics of cultured mast cells, including surface antigens which are shared with lymphocytes and mono‐ nuclear phagocytes. These results imply a relationship between the transforming gene product and the mast cell growth factor interleukin 3.

Original language	English (US)
Pages (from-to)	1136-1141
Number of pages	6
Journal	European Journal of Immunology
Volume	15
Issue number	11
DOIs	https://doi.org/10.1002/eji.1830151113
State	Published - 1985

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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abstract = "Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines express characteristics of cultured mast cells, including surface antigens which are shared with lymphocytes and mono‐ nuclear phagocytes. These results imply a relationship between the transforming gene product and the mast cell growth factor interleukin 3.",

author = "Siegel, {Michael L.} and Randy Horwitz and Morris, {Timothy D.} and {Di Venere}, {Raina M.} and Siden, {Edward J.}",

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AU - Siegel, Michael L.

AU - Horwitz, Randy

AU - Morris, Timothy D.

AU - Di Venere, Raina M.

AU - Siden, Edward J.

PY - 1985

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AB - Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines express characteristics of cultured mast cells, including surface antigens which are shared with lymphocytes and mono‐ nuclear phagocytes. These results imply a relationship between the transforming gene product and the mast cell growth factor interleukin 3.

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Isolation and characterization of Abelson murine leukemia virus‐transformed mast cell lines from midgestation embryonic placenta

Abstract

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