TY - JOUR
T1 - Isolation and characterization of a partial cDNA for a human sialyltransferase
AU - Lance, Peter
AU - Lau, Karen M.
AU - Lau, Joseph T.Y.
N1 - Funding Information:
IThis work was supported by a Veterans Administration grant c;M38193 from the National Institutes of Health
PY - 1989/10/16
Y1 - 1989/10/16
N2 - A probe generated from the coding sequence of the rat hepatic β-galactoside α2,6-sialyltransferase was used to screen a human cDNA library constructed of human submaxillary gland mRNA lambda gt-11. We report the isolation and characterization of a human cDNA, HSM-ST1, that is putatively the human homolog of the β-galactoside α2,6-sialyltransferase. The largest human clone contains a 1.3 kb cDNA insert and is predicted to encompass 75% of the coding sequence as well as a small portion of the 3′ untranslated region. Comparative analysis of this insert with the rat hepatic α2,6-sialyltransferase sequence indicates 79% nucleotide similarity between the two sequences in the predicted coding region. On the amino acid level, the degree of conservation is 86%. Substantial sequence similarity is observed in the 3′-untranslated region between the rat and human sequences as well. S1 nuclease analysis was performed to demonstrate the expression of HSM-ST1 transcripts in the human hepatoma cell line, HepG2, and in the human colonic adenocarcinoma cell lines, LS174T.
AB - A probe generated from the coding sequence of the rat hepatic β-galactoside α2,6-sialyltransferase was used to screen a human cDNA library constructed of human submaxillary gland mRNA lambda gt-11. We report the isolation and characterization of a human cDNA, HSM-ST1, that is putatively the human homolog of the β-galactoside α2,6-sialyltransferase. The largest human clone contains a 1.3 kb cDNA insert and is predicted to encompass 75% of the coding sequence as well as a small portion of the 3′ untranslated region. Comparative analysis of this insert with the rat hepatic α2,6-sialyltransferase sequence indicates 79% nucleotide similarity between the two sequences in the predicted coding region. On the amino acid level, the degree of conservation is 86%. Substantial sequence similarity is observed in the 3′-untranslated region between the rat and human sequences as well. S1 nuclease analysis was performed to demonstrate the expression of HSM-ST1 transcripts in the human hepatoma cell line, HepG2, and in the human colonic adenocarcinoma cell lines, LS174T.
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U2 - 10.1016/0006-291X(89)91706-3
DO - 10.1016/0006-291X(89)91706-3
M3 - Article
C2 - 2803295
AN - SCOPUS:0024452913
SN - 0006-291X
VL - 164
SP - 225
EP - 232
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -