Isolation and characterization of 17 microsatellite loci for the house finch (Carpodacus mexicanus)

Kevin P. Oh; Alexander V. Badyaev

doi:10.1111/j.1755-0998.2009.02555.x

Isolation and characterization of 17 microsatellite loci for the house finch (Carpodacus mexicanus)

Kevin P. Oh, Alexander V. Badyaev

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1 Scopus citations

Abstract

The house finch (Carpodacus mexicanus) has emerged recently as a model species in studies of sexual selection, reproductive physiology, population genetics, and epizootic disease ecology. Here we describe 17 highly polymorphic microsatellite loci for this species. In a sample of 36 individuals, we observed an average of 16 alleles per locus and heterozygosity ranged from 0.61 to 0.97. One locus showed significant deviation from Hardy-Weinberg proportions, but no significant gametic disequilibrium was observed among any of the loci. Amplification by polymerase chain reaction was optimized under similar parameters across loci, thereby facilitating multiplexing and rapid multilocus genotyping.

Original language	English (US)
Pages (from-to)	1029-1031
Number of pages	3
Journal	Molecular Ecology Resources
Volume	9
Issue number	3
DOIs	https://doi.org/10.1111/j.1755-0998.2009.02555.x
State	Published - May 2009

Keywords

Carduelinae
Extra-pair paternity
Genetic complementarity
Relatedness

ASJC Scopus subject areas

Biotechnology
Ecology, Evolution, Behavior and Systematics
Genetics

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10.1111/j.1755-0998.2009.02555.x

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abstract = "The house finch (Carpodacus mexicanus) has emerged recently as a model species in studies of sexual selection, reproductive physiology, population genetics, and epizootic disease ecology. Here we describe 17 highly polymorphic microsatellite loci for this species. In a sample of 36 individuals, we observed an average of 16 alleles per locus and heterozygosity ranged from 0.61 to 0.97. One locus showed significant deviation from Hardy-Weinberg proportions, but no significant gametic disequilibrium was observed among any of the loci. Amplification by polymerase chain reaction was optimized under similar parameters across loci, thereby facilitating multiplexing and rapid multilocus genotyping.",

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AU - Oh, Kevin P.

AU - Badyaev, Alexander V.

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AB - The house finch (Carpodacus mexicanus) has emerged recently as a model species in studies of sexual selection, reproductive physiology, population genetics, and epizootic disease ecology. Here we describe 17 highly polymorphic microsatellite loci for this species. In a sample of 36 individuals, we observed an average of 16 alleles per locus and heterozygosity ranged from 0.61 to 0.97. One locus showed significant deviation from Hardy-Weinberg proportions, but no significant gametic disequilibrium was observed among any of the loci. Amplification by polymerase chain reaction was optimized under similar parameters across loci, thereby facilitating multiplexing and rapid multilocus genotyping.

KW - Carduelinae

KW - Extra-pair paternity

KW - Genetic complementarity

KW - Relatedness

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Isolation and characterization of 17 microsatellite loci for the house finch (Carpodacus mexicanus)

Abstract

Keywords

ASJC Scopus subject areas

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