TY - JOUR
T1 - Is what you eat and drink safe? Detection and identification of microbial contamination in foods and water
AU - Lloyd, Christopher R.
AU - Cleary, Florine C.
AU - Kim, Hea Young
AU - Estes, Cory R.
AU - Duncan, Andrew G.
AU - Wade, Brad D.
AU - Ellis, Walther R.
AU - Powers, Linda S.
PY - 2003
Y1 - 2003
N2 - Current technologies for assessing the microbial load in food and water require cellular outgrowth to increase the cell count to a detectable level. This step therefore makes real-time assessment of microbial loads impossible. To circumvent this problem, we have developed a high-sensitivity, multiwavelength fluorescence detector and signal- collection and processing software. This detection system enables one to distinguish between abiotic matter, sporulated bacteria, vegetative bacteria, dead bacteria, and other biomaterials containing aromatic amino acids. Our detection limit is in the 10-100 cell/cm2 range (or per cm3 if bulk aqueous samples are used), and there is no sample contact. To supplement this detection system, we have developed a variety of ligands, including hemin derivatives, peptides, glycoconjugates, and siderophores, for cell capture to circumvent problems associated with antibodies. These materials are tethered to disposable surfaces (glass, plastics) in arrays for use in capturing biomaterials (e.g., live bacterial cells, dead cells, spores, and toxins); identification is based on which sector of an array it binds to. The detection system can serve as a reader for these coated materials, and variants have been tested for use in scanning food surfaces and food wash water.
AB - Current technologies for assessing the microbial load in food and water require cellular outgrowth to increase the cell count to a detectable level. This step therefore makes real-time assessment of microbial loads impossible. To circumvent this problem, we have developed a high-sensitivity, multiwavelength fluorescence detector and signal- collection and processing software. This detection system enables one to distinguish between abiotic matter, sporulated bacteria, vegetative bacteria, dead bacteria, and other biomaterials containing aromatic amino acids. Our detection limit is in the 10-100 cell/cm2 range (or per cm3 if bulk aqueous samples are used), and there is no sample contact. To supplement this detection system, we have developed a variety of ligands, including hemin derivatives, peptides, glycoconjugates, and siderophores, for cell capture to circumvent problems associated with antibodies. These materials are tethered to disposable surfaces (glass, plastics) in arrays for use in capturing biomaterials (e.g., live bacterial cells, dead cells, spores, and toxins); identification is based on which sector of an array it binds to. The detection system can serve as a reader for these coated materials, and variants have been tested for use in scanning food surfaces and food wash water.
KW - Microbial detection
KW - Microbial food contamination
KW - Microbial identification
KW - Microbial water contamination
UR - http://www.scopus.com/inward/record.url?scp=2442653672&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442653672&partnerID=8YFLogxK
U2 - 10.1109/JPROC.2003.813571
DO - 10.1109/JPROC.2003.813571
M3 - Review article
AN - SCOPUS:2442653672
SN - 0018-9219
VL - 91
SP - 908
EP - 913
JO - Proceedings of the Institute of Radio Engineers
JF - Proceedings of the Institute of Radio Engineers
IS - 6
ER -