Iron loaded ferritin secretion and inhibition by CI-976 in Aedes aegypti larval cells

Dawn L. Geiser, Meng Chieh Shen, Jonathan J. Mayo, Joy J. Winzerling

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Ferritin is a multimer of 24 subunits of heavy and light chains. In mammals, iron taken into cells is stored in ferritin or incorporated into iron-containing proteins. Very little ferritin is found circulating in mammalian serum; most is retained in the cytoplasm. Female mosquitoes, such as Aedes aegypti (yellow fever mosquito, Diptera), require a blood meal for oogenesis. Mosquitoes receive a potentially toxic level of iron in the blood meal which must be processed and stored. We demonstrate by 59Fe pulse-chase experiments that cultured A. aegypti larval CCL-125 cells take up iron from culture media and store it in ferritin found mainly in the membrane fraction and secrete iron-loaded ferritin. We observe that in these larval cells ferritin co-localizes with ceramide-containing membranes in the absence of iron. With iron treatment, ferritin is found associated with ceramide-containing membranes as well as in cytoplasmic non-ceramide vesicles. Treatment of CCL-125 cells with iron and CI-976, an inhibitor of lysophospholipid acyl transferases, disrupts ferritin secretion with a concomitant decrease in cell viability. Interfering with ferritin secretion may limit the ability of mosquitoes to adjust to the high iron load of the blood meal and decrease iron delivery to the ovaries reducing egg numbers.

Original languageEnglish (US)
Pages (from-to)352-363
Number of pages12
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Issue number4
StatePublished - Apr 2009


  • Aedes aegypti
  • CI-976
  • Ferritin
  • Iron
  • Mosquito
  • Secretion

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology


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